饮水型砷暴露人群的血液芳香烃受体和细胞色素P450 1A1 mRNA表达水平与心电图校正QT间期的关系  

Relationship of blood aryl hydrocarbon receptor mRNA and cytochrome P450 1A1 mRNA expression with corrected QT interval among residents exposed to arsenic via drinking water

作　　者：崔娜[1] 李艳红[1] 刘建平[2] 武克恭[1] 夏雅娟[1] 

机构地区：[1] 内蒙古自治区地方病防治研究中心,呼和浩特010031 [2] 包头市中心医院

出　　处：《中华心血管病杂志》2016年第3期260-264,共5页Chinese Journal of Cardiology

基　　金：国家自然科学基金（s1360414）

摘　　要：目的 探讨饮水型砷暴露人群血液的芳香烃受体（AhR） mRNA和细胞色素P450（CYP） 1A1 mRNA的表达水平与心电图校正QT间期之间的关系.方法 2012年12月至2015年1月,选取内蒙古自治区巴彦淖尔市的砷暴露地区作为调查点,将在此居住10年以上的居民作为调查对象.将饮用水的砷浓度范围为0.8～824.7 μg/L的233名居民分为4组：对照组（饮水砷浓度＜10.0 μg/L,共55名）,低剂量组（饮水砷浓度10.0～99.9 μg/L,共47名）,中剂量组（饮水砷浓度100.0～199.9 μg/L,共45名）,高剂量组（饮水砷浓度≥200.0 μg/L,共86名）.对研究对象进行流行病学调查,并采用实时荧光定量PCR技术,检测血液AhR mRNA和CYP1A1 mRNA的表达水平,分析其与校正QT间期之间的关系.结果 （1）对照组、低剂量组和中剂量组之间的血液AhR mRNA和CYP1A1 mRNA表达水平差异均无统计学意义（P均＞0.05）;高剂量组的血液AhR mRNA[3.18＆#215;10-3（2.42 ＆#215;10-3,4.45＆#215;10-3）比2.30＆#215;10-3 （1.53＆#215;10-3,3.20＆#215;10-3）,P＜0.05]和CYP1 A1mRNA [1.58＆#215;10-3（0.80＆#215;10-3,2.73＆#215;10-3）比1.00＆#215;10-3 （0.59＆#215;10-3,2.09＆#215;10-3）,P ＜0.05]表达水平均高于对照组.（2）校正QT间期延长者与正常者的AhR mRNA表达水平差异无统计学意义[2.89＆#215;10-3（1.90＆#215;10-3,3.71 ＆#215;10-3）比2.58＆#215;10-3（1.85＆#215;10-3,3.57＆#215;10-3）,P＞0.05];校正QT间期延长者的CYP1 A1 mRNA表达水平高于校正QT间期正常者[1.50＆#215;10-3（0.78＆#215;10-3,2.63＆#215;10-3）比1.16＆#215;10-3 （0.64＆#215;10-3,2.36＆#215;10-3）,P＜0.05].（3）窦性心动过速或过缓者与心率正常者的AhR mRNA表达水平差异无统计学意义[2.89＆#215;10-3（1.71＆#215;10-3,4.45＆#215;10-3）比2.64＆#215;10-3（1.86＆#215;10-3,3.52＆#215;10-3）,P＞0.05].窦性心动过速或过缓者的CYP1A1 mRNA表达水平高于心率正常者[1.47＆#215;10-3 （0.87＆#215;10-3,2.7Objective To investigate the relationship between blood aryl hydrocarbon receptor（AhR） mRNA and cytochrome P450（CYP） 1A1 mRNA expression and corrected QT interval among residents exposed to arsenic via drinking water.Methods Arsenic exposure area in Bayannao’ er city of Nei Monggol Autonomous Region was selected as the survey point,and the residents living more than 10 years in this area were investigated from December 2012 to January 2015.A total of 233 residents were divided into four groups according to drinking water arsenic concentration （ranged from 0.8 to 824.7 μg/L）：control group （drinking water arsenic concentration 〈 10.0 μg/L,n =55）,low exposure group （drinking water arsenic concentration 10.0-99.9 μg/L,n =47）,middle exposure group （drinking water arsenic concentration 100.0-199.9 μg/L,n =45）,high exposure group （drinking water arsenic concentration ≥ 200.0 μg/L,n =86）.Epidemiological investigation was performed.Real-time PCR technology was used to detect the expression levels of blood AhR mRNA and CYP1A1 mRNA,and the relationship between expression levels of blood AhR mRNA and CYP1A1 mRNA and corrected QT interval was analyzed.Results （1） Blood AhR mRNA and CYP1A1 mRNA expression levels were similar among control group,low exposure group and middle exposure group （all P 〉0.05） while blood AhR mRNA （3.18 ＆#215; 10-3（2.42 ＆#215; 10-3,4.45 ＆#215; 10-3） vs.2.30 ＆#215; 10-3 （1.53 ＆#215; 10-3,3.20 ＆#215; 10-3）,p 〈 0.05） and CYP1A1 mRNA （1.58 ＆#215; 10-3 （0.80 ＆#215; 10-3,2.73 ＆#215; 10-3） vs.1.00 ＆#215; 10-3 （0.59 ＆#215; 10-3,2.09 ＆#215; 10-3）,p 〈 0.05） expression levels were significantly higher in high dose group than in control group.（2） AhR mRNA expression level was similar between residents with longer corrected QT interval and residents with normal corrected QT interval （2.89 ＆#215; 10-3 （1.90 ＆#215;10-3,3.71 ＆#215; 10-3） vs.2.58 ＆#215; 10-3（1.85 ＆#215; 10-3,3.57 ＆#215; 10-3�

关 键 词：砷 心电描记术 烃类 芳香 细胞色素P450酶系统 

分 类 号：R540.41[医药卫生—心血管疾病] R599.1[医药卫生—内科学]