多指标综合评分法优选参芪复方颗粒提取工艺及其热稳定性考察  

Optimization of Extraction Process of Shenqi Compound Granules with Multi-index Comprehensive Evaluation Method and Investigation of Its Thermal Stability

作　　者：单丽芳[1] 杨红梅[1] 曹蕾[1] 袁彩[1] 张林[1] 高天[2] 宋英[2] 

机构地区：[1]成都中医药大学,成都610075 [2]成都中医药大学附属医院,成都610072

出　　处：《中国实验方剂学杂志》2016年第7期24-27,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省科技支撑项目(2014SZ0140)

摘　　要：目的:优选参芪复方颗粒的水提工艺条件,并考察不同因素下提取液中皂苷类成分含量的变化。方法:采用HPLC-ELSD测定人参皂苷Rg1,Re,Rb1和黄芪甲苷的含量,流动相水-乙腈梯度洗脱,流速0.8 m L·min-1,气体流量2.6 L·min-1,漂移管温度102℃。以皂苷类含量及干膏得率的综合评分为指标,在单因素试验基础上,通过正交试验考察料液比、提取时间、提取次数对参芪复方颗粒水提工艺的影响;通过测定不同影响因素下参芪复方颗粒水提液及浓缩液中皂苷类含量的变化,考察该制剂的热稳定性。结果:最佳提取工艺为加10倍量水提取3次,每次40 min;人参皂苷Rg1,Re,Rb1和黄芪甲苷提取量分别为1.556,2.155,2.168,0.718 mg·g-1。参芪复方颗粒制剂工艺过程中受热温度和时间是影响其皂苷类成分含量变化的主要因素之一。结论:参芪复方颗粒在制备工艺过程中应避免高温及长时间受热,在浓缩中应采用减压浓缩法,以降低人参皂苷类成分的降解。优选的水提工艺合理可行、重复性好,适用于该制剂的工业化生产。Objective： To optimize water extraction process of Shenqi compound granules and investigate effects of different factor on the content of saponins in water extract and concentrated solution. Method：HPLC-ELSD was employed to determine contents of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and astragaloside Ⅳ at the same time. Based on single factor tests,taking solid-liquid ratio,extraction time and times as factors, orthogonal test was adopted to optimized extraction process with composite score of the content of saponins and yield of dry extract as index. Stability of Shenqi compound granules was investigated by determining the content of saponins in water extract and concentrated solution under different factor. Result： Optimum extraction process was as follows： extracted thrice with 10 times the amount of water,40 min for each time.Extracting amounts of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and astragaloside Ⅳ were 1. 556,2. 155,2. 168,0. 718 mg ·g- 1. Heating temperature and time was main factors affecting content change of saponins in Shenqi compound granules. Conclusion： High temperature and long time heating should avoid in preparation process of Shenqi compound granules,vacuum concentration method and vacuum drying method should be adopted to reduce degradation rate of ginsenosides. This optimized extraction process is feasible and suitable for industrial production of Shenqi compound granules.

关 键 词：参芪复方颗粒 热稳定性 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 黄芪甲苷 干膏得率 

分 类 号：R283.6[医药卫生—中药学] R944.2[医药卫生—中医学]