多指标正交试验优选双黄二仙颗粒的提取工艺  

Optimization of Extraction Technology of Shuanghuang Erxian Granules by Orthogonal Test

作　　者：杨红梅[1] 曹蕾[1] 单丽芳[1] 宋英[2] 涂翔[2] 钟森[2] 

机构地区：[1]成都中医药大学,成都610075 [2]成都中医药大学附属医院,成都610072

出　　处：《中国实验方剂学杂志》2016年第7期28-30,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省中医药管理局青年基金项目(2014K108);成都市科技局科技惠民应用示范项目(2014-HM02-00014-SF);成都中医药大学附属医院科技发展基金项目(2013-D-YY-14)

摘　　要：目的:优选双黄二仙颗粒的提取工艺,为该制剂的工业化生产提供参考。方法:采用HPLC测定黄芪甲苷、沙苑子苷含量,流动相分别为乙腈-水(35∶65)和流动相乙腈-0.2%磷酸溶液(21∶79)。以黄芪甲苷、沙苑子苷提取量和浸膏得率为综合评价指标,通过正交试验考察加水量、提取时间和提取次数对双黄二仙颗粒提取工艺的影响。结果:最佳提取工艺为加8倍量水提取2次,每次1 h;黄芪甲苷、沙苑子苷平均提取量分别为1.084,1.343 mg·g-1,浸膏得率平均值28.48%,提取次数对提取工艺具有显著性影响。结论:优选的提取工艺提取率高、稳定性好,可为双黄二仙颗粒的工业化生产工艺提供指导。Objective： To optimize extraction technology of Shuanghuang Erxian granules. Method：HPLC was employed to determine contents of astragaloside Ⅳ and complanatuside in Shuanghuang Erxian granules with mobile phases of acetonitrile-water（ 35 ∶ 65） and acetonitrile-0. 2% phosphoric acid solution（ 21 ∶ 79）,respectively. Taking contents of astragaloside Ⅳ and complanatuside, yield of dry extract as comprehensive evaluation index,orthogonal test was used to investigate effects of water amount,extracting time and times on extraction process of Shuanghuang Erxian granules. Result： Optimum extraction condition was as follows：extracted twice with 8 times the amount of water,1 hour for per time. Extracting amounts of astragaloside Ⅳ and complanatuside were 1. 084,1. 343 mg·g- 1,yield of dry extract was 28. 48%,extraction time had a significant effect on extraction technology of Shuanghuang Erxian granules. Conclusion： This optimized extraction condition is stable and feasible,it can provide guidance for industrial production of Shuanghuang Erxian granules.

关 键 词：黄芪甲苷 沙苑子苷 浸膏得率 双黄二仙颗粒 综合评分 

分 类 号：R283.6[医药卫生—中药学] R944.2[医药卫生—中医学]