金水涤痰口服液的提取工艺考察  

Investigation of Extraction Process of Jinshui Ditan Oral Liquid

作　　者：曹蕾[1] 单丽芳[1] 杨红梅[1] 宋英[2] 

机构地区：[1]成都中医药大学,成都610075 [2]成都中医药大学附属医院,成都610072

出　　处：《中国实验方剂学杂志》2016年第7期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：成都中医药大学附属医院基金项目(2010-D-YY-03)

摘　　要：目的：优选金水涤痰口服液的提取工艺,为该制剂的工业化生产提供参考。方法：以挥发油提取率为指标,采用单因素试验考察芳香水的提取工艺。以橙皮苷、淫羊藿苷提取量及干膏收率的综合评分为指标,通过L9（34）正交试验考察加水量、煎煮时间、煎煮次数对提取效果的影响。采用HPLC测定橙皮苷和淫羊藿苷的含量,流动相0.1%磷酸（A）-乙腈（B）梯度洗脱（0-11 min,77%A;11-13 min,77%-70%A;13-30 min,70%A;30-32 min,70%-77%A）,检测波长283 nm,流速1.0 m L·min-1,柱温30℃。结果：芳香水提取工艺为加10倍量水,以2 s/滴的速度蒸馏提取4 h,再以相同蒸馏速度将芳香水重蒸馏提取2 h,得2次蒸馏芳香水的体积约为药材质量的1.1倍。各因素对水提工艺的影响顺序为煎煮次数〉煎煮时间〉加水量,最佳提取工艺为加8倍量水煎煮3次,每次1.5 h;橙皮苷、淫羊藿苷提取量分别为27.77,15.30 mg·g-1,干膏收率40.13%。结论：优选的提取工艺稳定、可行,可为金水涤痰口服液的工业化生产提供参考。Objective： To optimize extraction technology of Jinshui Ditan oral liquid. Method： Single factor tests were adopted to optimize extraction technology of aromatic water with extraction rate of volatile oil as index. With composite score of extracting amounts of hesperidin and icariin, yield of dry extract as index,orthogonal test was adopted to investigate effects of the amount of water,decocting time and times on extraction process. HPLC was employed to determine contents of hesperidin and icariin. Result： Aromatic water extraction process was distilled 4 hours with 10-fold water at a speed of 2 seconds per drop, then distilled 2 hours from aromatic water at the same speed, the final volume of distilled fragrance water was about 1. 1 times weight of medicine. Water extraction technology was as follows： decocted thrice with 8-fold water,each time for 1. 5 hour;extracting amounts of hesperidin and icariin were 27. 77,15. 30 mg ·g- 1,yield of dry extract was 40. 13%.Conclusion： These optimized processes are stable and feasible, they can provide a reference for industrial production of Jinshui Ditan oral liquid.

关 键 词：芳香水 淫羊藿苷 橙皮苷 综合评分法 干膏收率 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]