须根腐解对丹参根际土壤基本理化性质的影响  

Effect of Fibrous Roots Decomposition on Basic Physicochemical Properties in Rhizosphere Soil of Salviae Miltiorrhizae Radix et Rhizoma

作　　者：刘伟[1] 吕海花 周洁[1] 王晓[1] 耿岩玲[1] 刘建华[1] 李奉胜 

机构地区：[1]山东省中药质量控制技术重点实验室山东省分析测试中心,济南250014 [2]莱芜紫光生态园有限公司,山东莱芜271100

出　　处：《中国实验方剂学杂志》2016年第7期42-45,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81102749;81303161);山东省科技发展计划项目(2012GSF11901;2015GSF119021)

摘　　要：目的:研究须根腐解对丹参根际土壤基本理化性质的影响。方法:利用电感耦合等离子体发射光谱法(ICPAES)测定土壤中营养元素的含量,采用HPLC测定丹参中活性成分的含量,流动相0.2%乙酸水溶液-乙腈梯度洗脱。结果:添加20 g须根处理土壤中Ca,Fe,K,Zn和N元素的含量分别比对照组(CK)降低了1.33%,1.95%,0.15%,5.81%和15.38%,添加40 g须根处理分别比CK降低了4.10%,3.62%,2.47%,5.81%和53.85%;土壤中Cu,Mn和P元素的含量与丹参酮Ⅰ的含量呈显著正相关;K元素含量与丹参酮Ⅰ,丹参酮ⅡA的含量呈显著正相关;Ca元素含量与丹参地上部分干重呈显著正相关,土壤中Zn元素含量与丹参根鲜重呈显著正相关,Fe元素与丹参根中迷迭香酸含量呈显著负相关。结论:丹参根际土壤中矿质元素含量与其产量或品质存在一定相关性。须根腐解产生的一系列生物、化学作用可能影响到丹参根际土壤中矿质元素含量,从而影响丹参的产量和品质。Objective： Change of basic physicochemical properties in rhizosphere soil was studied while fibrous roots of Salviae Miltiorrhizae Radix et Rhizoma were decayed naturally. Method： Contents of nutrient elements were measured by ICP-AES and contents of active ingredients in Salviae Miltiorrhizae Radix et Rhizoma were measured by HPLC. Result： Compared with the control group（ CK）,contents of Ca,Fe,K,Zn and N in the treatment 1 group（ added fibrous roots of 20 g） decreased by 1. 33%,1. 95%,0. 15%,5. 81% and15. 38%; they decreased by 4. 10%,3. 62%,2. 47%,5. 81% and 53. 85% in the treatment 2 group（ added fibrous roots of 40 g）,respectively. Contents of Cu,Mn and P in soil were positively related to the content of tanshinoneⅠ significantly; the content of K was positively related to contents of tanshinoneⅠ and tanshinon ⅡA significantly in the same time; the content of Ca was positively related to dry weight on the ground of Salviae Miltiorrhizae Radix et Rhizoma significantly; the content of Zn was positively related to root fresh weight significantly; the content of Fe was negatively related to the content of rosemary acid significantly. Conclusion：Contents of mineral elements in rhizosphere soil have a certain relation to yield or quality of Salviae Miltiorrhizae Radix et Rhizoma. Fibrous roots decomposition can affect contents of mineral elements in rhizosphere soil of this herb,which is the reason that its yield and quality changed under different treatment.

关 键 词：丹参 须根腐解 活性成分 产量 无机元素 

分 类 号：R282.2[医药卫生—中药学] R817.92[医药卫生—中医学]