见血青HPLC指纹图谱研究  

Fingerprints of Liparis nervosa by HPLC

作　　者：赵颖[1] 胡少南[1] 宋丹 王昌华[3] 郑一敏[1] 胥秀英[1] 

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054 [2]重庆市药品技术审评认证中心,重庆400014 [3]重庆市中药研究院,重庆400065

出　　处：《中国实验方剂学杂志》2016年第7期65-69,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市教委科学技术研究项目(KJ110802);重庆理工大学科研启动基金项目(2010ZD07)

摘　　要：目的:建立见血青药材的高效液相色谱(HPLC)指纹图谱,为科学评价及控制见血青质量提供依据。方法:采用HPLC法,色谱条件为Welchrom-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%三乙胺水溶液(10%磷酸水溶液调p H2.5)进行梯度洗脱,流速1.0 m L·min-1,检测波长254 nm,柱温30℃。建立10个不同产地见血青指纹图谱,采用"中药色谱指纹图谱相似度评价系统2004A版"软件进行相似度评价,并采用SPSS 19.0统计软件进行聚类分析。结果:从指纹图谱中标定了28个共有峰,并指认了其中6个共有峰(3个生物碱,3个黄酮),经相似度评价,10个产地中有8个产地的见血青与共有模式图谱比较相似度均在0.9以上,整体相似度好,聚类分析结果与相似度评价结果基本一致。而广东梅县和四川雅安两产地药材的相似度仅为0.173和0.320,这些差异可能是因气候条件、地域差异、采收季节的不同所致。结论:该方法灵敏度高、重复性好,简单可靠,可用于见血青药材的质量评价与控制。Objective： To establish HPLC fingerprints of Liparis nervosa and provide the basis for scientific evaluation and quality control of L. nervosa. Method： HPLC method was used on Welchrom-C18（ 4. 6mm × 250 mm,5 μm） chromatographic column,with Acetonitrile-0. 2% triethylamine solution（ 10% phosphoric acid solution,p H adjusted to 2. 5） as gradient elution. The wavelength was detected as 254 nm; the flow rate was1. 0 m L·min- 1,and the column temperature was 30 ℃. The HPLC fingerprints were established for the L. nervosa from 10 different origins. At the same time,the similarity evaluation system for chromatographic fingerprint of TCM（ 2004 A version） was applied for similarity evaluation. Besides,cluster analysis was done by using SPSS 19. 0software. Result： Twenty-eight common peaks were calibrated from the HPLC fingerprints of L. nervosa,six of which were identified by reference substances（ 3 alkaloids and 3 flavonoids）. In the similarity evaluation,L. nervosa from 8 of 10 origins had a similarity ratio of more than 0. 9 when compared with the mutual mode HPLC fingerprints. The overall similarity was good,and the cluster analysis result was basically the same as the similarity evaluation result. However,the similarity ratio of the materials from Mei county in Guangdong was only 0. 173 and that of Ya＇an in Sichuan was 0. 320. These differences may be caused due to weather conditions, regional differences and different harvest seasons. Conclusion： This method is highly sensitive,reproducible,simple and reliable,and can be used for quality control of L. nervosa.

关 键 词：见血青 高效液相色谱 指纹图谱 相似度评价 聚类分析 

分 类 号：R284.1[医药卫生—中药学]