HPLC同时测定人参药性菌质中8种皂苷含量  

Simultaneous Determination of 8 Saponins in Ginseng Medicinal Fungal Substance by HPLC

作　　者：朱莹莹[1] 刘鑫[1] 贾艾玲[1] 王琳[1] 邱智东[1] 朱凯[1] 

机构地区：[1]长春中医药大学,长春130117

出　　处：《中国实验方剂学杂志》2016年第7期96-99,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2012BAI29B05);吉林省科技厅项目(20130206025YY)

摘　　要：目的:建立一种可以同时测定人参药性菌质中8种人参皂苷(人参皂苷Rg1,人参皂苷Re,人参皂苷Rf,人参皂苷Rh1,人参皂苷Rb1,人参皂苷Ro,人参皂苷Rb2,人参皂苷Rd)含量的HPLC方法。方法:采用高效液相法,Inertsil ODS-SP色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.4%磷酸水梯度洗脱,流速1 m L·min-1,检测波长203 nm,柱温30℃,测定人参药性菌质8种皂苷的含量。结果:8种单体皂苷在100 min内可良好分离,线性关系良好(R2>0.999 7),平均加样回收率分别为100.15%,101.54%,101.87%,100.88%,102.10%,102.84%,101.38%,101.23%,RSD分别为1.6%,2.3%,1.9%,2.1%,1.5%,0.8%,2.7%,1.6%。结论:该研究提供了一种能方便、简单、准确、重复性好的HPLC分析人参药性菌质中8种人参皂苷含量的方法,可为人参药性菌质成分转化研究的质量标准制定提供科学依据。Objective： To establish an HPLC method for simultaneous determination of ginsenoside Rg1,ginsenoside Re, ginsenoside Rf, ginsenoside Rh1,ginsenoside Rb1,ginsenoside Ro,ginsenoside Rb2,and ginsenoside Rd in ginseng medicinal fungal substance. Method： High performance liquid chromatography was carried out on Inertsil ODS-SP chromatographic column（ 4. 6 mm × 250 mm,5 μm） with acetonitrile-0. 4%phosphate as the mobile phase for gradient elution. The flow rate was 1 m L·min- 1,and detection wavelength was203 nm. The column temperature was at 30 ℃. Result： Eight kinds of monomer saponins could be separated well in 100 min, with good linear relationship（ R2 〉0. 999 7）. The average recovery rate was 100. 15%（ RSD1. 6%）,101. 54%（ RSD 2. 3%）,101. 87%（ RSD 1. 9%）,100. 88%（ RSD 2. 1%）,102. 10%（ RSD1. 5%）,102. 84%（ RSD 0. 8%）,101. 38%（ RSD 2. 7%）,and 101. 23%（ RSD 1. 6%）. Conclusion： The HPLC method is accurate,reliable and reproducible to analyze 8 saponins in ginseng medicinal fungal substance,which can provide the scientific basis for formulating the quality standard of the research on composition transformation of the ginseng medicinal fungal substance.

关 键 词：人参药性菌质 人参皂苷RG1 人参皂苷RE 人参皂苷Rf 人参皂苷RH1 

分 类 号：R284.1[医药卫生—中药学]