microRNA-21在急性肾损伤小鼠肾脏中的表达及意义  

Expression and significance of microRNA -21 in acute kidney injury renal of mice

作　　者：邓旭[1] 李志辉[1] 康志娟[1] 王颖[1] 夏团红 丁妞[1] 银燕[1] 

机构地区：[1]湖南省儿童医院肾内科湖南省儿科医学研究所肾脏病研究室,长沙410007

出　　处：《中华实用儿科临床杂志》2016年第6期441-444,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的探究microRNA-21（miR-21）在急性肾损伤（AKI）小鼠肾脏再灌注不同时间点的表达及意义。方法将C57BL／6J小鼠分为3大组：对照组（C组）、假手术组（s组）和缺血再灌注组（IR组），后2组又根据再灌注时间点各分为9个亚组。全自动生化仪检测血肌酐（Scr）、血尿素氮（BUN）水平；HE染色观察肾脏病理损害情况；肾小管间质病理评分计量肾脏病理损害程度；实时荧光定量（RT）-PCR检测miR-21、丝裂原活化蛋白激酶激酶3（MKK3）表达；免疫组织化学染色法检测肾组织MKK3表达。结果IR组再灌注之后Scr、BUN、肾脏病理损害评分水平逐渐增高，24h达峰值，之后逐渐下降，IR组与S组同时间点亚组间在再灌注3—168h的Scr、BUN、肾小管间质病理损害差异均有统计学意义（P均〈0．01）；miR-21在肾脏缺血再灌注后表达逐渐上升，24h达到峰值并稳定在此高值水平，与肾小管间质病理损伤呈正相关（r=0．969，P〈0．05）；IR组MKK3mRNA及蛋白质表达在再灌注后逐渐上升，于24h达到最高值，之后逐渐下降，IR组与S组3～168h各相同时间点亚组MKK3mRNA表达水平差异均有统计学意义（P均〈0．01）；在0～168h的MKK3蛋白质表达水平差异也有统计学意义（P〈0．01）。结论miR-21在肾脏缺血再灌注后表达逐渐上升，24h达峰值并稳定在此水平，且与肾小管间质病理损伤呈正相关，这种作用可能与miR-21调控MKK3相关。Objective To investigate the expression and significance of microRNA -21 （miR- 21 ） in acute kidney injury mice model at the different time points following ischemic/reperfusion. Methods C57BL/6J mice were divided into 3 major groups ： the control group （ C group）, sham operation group （ S group） and ischemia - reperfusion group （ IR group）. Later 2 groups were divided into 9 sub - groups respectively according to the time following reperfu- sion. Automatic biochemical analyzer detected serum creatinine （Scr）, blood urea nitrogen （BUN） level. HE staining detected renal pathological damage. Renal tubulointerstitial pathological score accessed pathological damage. Real time - PCR tested the expression of miR - 21 and mitogen - activated protein kinase kinase 3 （ MKK3 ） mRNA in renal respectively. Immunohistochemistry staining tested expression of MKK3. Results IR group＇s Scr,BUN levels gradually increased following reperfusion,24 h reached its peak, then gradually declined. The Scr, BUN level had statistically sig- nificant difference between IR group and S group at the same time subgroup from 3 h to 168 h following reperfusion（ all P 〈 0.01 ）. The change of kidney damage and pathological changes of interstitial and tubular injury score consensus with renal function, miR -21 increased gradually in renal ischemia after reperfusion,24 h peaked and then stabilized at this high level, miR- 21 was positively correlated with pathological tubulointerstitial injury from 0 h to 168 h after reperfu- sion （r = 0. 969 ,P 〈 0.05 ）. IR group＇s MKK3 mRNA and protein expression rose sharply following ischemia/reperfu- sion,24 h peaked,and then gradually decreased. From 3 h to 168 h,the expression of MKK3 mRNA and proteins had significant difference at each same time points subgroups between IR group and S group（ all P 〈 0.01 ）. Conclusions miR-21 increases gradually in renal ischemia after reperfusion,24 h peaked and then stabilized at this high level. miR- 21 is positively corre

关 键 词：MICRORNA-21 缺血再灌注损伤 急性肾损伤 丝裂原活化蛋白激酶激酶3 

分 类 号：R726.9[医药卫生—儿科]