超抗原SEB对角质形成细胞糖皮质激素受体的作用研究  

Effects of superantigen SEB on glucocorticoid receptor in human keratinocyte

作　　者：王为[1] 黄珂[1] 冉磊[1] 刘莲[1] 王玉娟[1] 李元朝[1] 何威[1] 王儒鹏[1] 

机构地区：[1]第三军医大学新桥医院皮肤风湿免疫科,重庆400037

出　　处：《免疫学杂志》2016年第4期283-287,293,共6页Immunological Journal

基　　金：国家自然科学基金面上项目(81271767);第三军医大学第二附属医院临床科研项目(2015YLC26)

摘　　要：目的探讨超抗原金黄色葡萄球菌肠毒素B(SEB)对人永生化角质形成细胞(Ha Ca T细胞)糖皮质激素受体表达及核转移的影响。方法采用不同浓度SEB作用于体外培养的Ha Ca T细胞,RT-PCR、Western blot分别检测Ha Ca T细胞糖皮质激素受体α、β(GRα、GRβ)m RNA、蛋白的表达;随后用SEB预处理Ha Ca T细胞,地塞米松再作用Ha Ca T细胞8 h后,免疫荧光法检测Ha Ca T细胞GRα细胞内分布情况。结果 SEB作用Ha Ca T细胞后,其GRαm RNA、蛋白表达无明显变化,GRβm RNA、蛋白表达随SEB的浓度增高呈上升趋势,且在SEB质量浓度为100 ng/ml时达到最高值。10-6mol/L地塞米松作用8 h后能够诱导Ha Ca T细胞GRα由胞浆向胞核转移,该效应能够维持到24 h。与地塞米松组Ha Ca T细胞内GRα分布出现向核内转移现象不同,地塞米松+SEB组部分细胞GRα分布仍局限于胞质,并未出现核转移现象。结论 SEB可能通过诱导角质形成细胞GRβ表达上调及抑制地塞米松诱导的GRα由胞浆向胞核转移参与炎症性皮肤病外用糖皮质激素抵抗。This study aimed to investigate the effects of superantigen staphylococcal enterotoxin B（SEB） onglucocorticoid receptor in human keratinocyte Ha Ca T cell line. Cultured Ha Ca T cells were stimulated with SEB atdifferent concentrations, and then the m RNA and protein levels of glucocorticoid receptor α and β（GRα and GRβ）were measured by RT-PCR and Western blot. After pretreated with SEB, Ha Ca T cells were incubated withdexamethasone for 8 h to observe the intracellular localization of GRα. Data showed that SEB could increase them RNA and protein levels of GRβ in a dose-dependent manner, reaching a maximum when the concentration ofSEB came to 100 ng/ml in Ha Ca T cells. Neither the m RNA nor protein level of GRα did not affected by SEB.Dexamethasone at the concentration of 10-6mol/L had the ability to induce the translocation of GRα from thecytoplasm to the nucleus. However, the ability was partly inhibited by SEB. In conclusion, SEB may contribute totopical glucocorticoid resistance by increasing GRβ expression and inhibiting dexamethasone-induced GRα nucleartranslocation in keratinocyte.

关 键 词：超抗原 SEB 糖皮质激素受体 糖皮质激素抵抗 

分 类 号：R96[医药卫生—药理学]