肺炎链球菌肺炎对肺部树突状细胞及CD4^+T细胞的影响  

Modulation of dendritic cells and CD4^+T cells in mouse lungs during Streptococcus pneumonia

作　　者：刘茹[1] 田琴琴[1] 张光莉[2] 邹心 周干[1] 陈明[1] 孟庆清[1] 董世访[1] 罗征秀[2] 

机构地区：[1]儿童发育疾病研究教育部重点实验室儿科学重庆市重点实验室儿童发育重大疾病国际科技合作基地,400014 [2]重庆医科大学附属儿童医院呼吸科

出　　处：《免疫学杂志》2016年第4期288-293,共6页Immunological Journal

基　　金：国家自然科学基金(81070015;81270086)

摘　　要：目的探讨肺炎链球菌肺炎对Balb/c小鼠肺部树突状细胞及CD4^+T细胞的影响。方法 6~8周Balb/c小鼠随机分为对照组及肺炎链球菌感染组,流式细胞术检测肺炎链球菌感染后2 d、5 d肺组织中CD103^+DCs、CD11b^+DCs、p DCs及CD4^+T细胞亚类水平。结果肺炎链球菌肺炎致肺组织CD103^+DCs及p DCs水平显著增高,与对照组比较差异有统计学意义(P<0.01),而肺组织CD11b^+DCs水平显著低于对照组。肺炎链球菌肺炎促进肺组织IL-17A^+CD4^+T细胞和Foxp3^+Tregs表达,与对照组比较,感染后2 d、5 d差异均有统计学意义(P<0.05),随着感染控制,Foxp3^+Tregs表达水平回降,感染后5 d Foxp3^+Tregs水平显著低于感染后2 d水平(7.3%±0.41%vs 9.38%±1.34%,P<0.01)。肺炎链球菌肺炎对肺部Th2表达水平无显著影响,感染后5 d IFN-γ^+CD4^+T细胞水平显著升高,与对照组、感染后2 d组比较,差异有统计学意义(P<0.01)。结论肺炎链球菌肺炎促进肺组织CD103^+DCs、p DCs表达,诱导CD4^+Th17、Tregs细胞分化发育。To investigate the alteration of dendritic cells（DCs） and CD4^＋T cells after Streptococcus pneumoniae（S. pneumonia） infection, 6-8 weeks Balb/c mice were divided into Streptococcus pneumonia infection and mockinfection groups. The levels of CD103^＋DC, CD11b^＋DC, p DC and the subsets of CD4^＋T cells in lungs were detectedby flow cytometry on 2^（nd） and 5^（th） day after infection. 2 and 5 days after infection, CD103^＋DC and p DC levels in S.pneumonia infection groups were significantly elevated（P〈0.01）, but the level of CD11b^＋DC was significantlydecreased（P〈0.05） as compared with mock infection groups. IL-17A^＋CD4^＋T cells and Foxp3^＋Tregs levels in S.pneumonia-infected group were increased compared with mock-infected group on 2ndand 5thday after infection（P〈0.05）; IL-4^＋CD4^＋T cells level was similar between mock and S. pneumonia infection group, while IFN-γ^＋CD4^＋Tcells were significantly increased 5 days post S. pneumonia infection when compared with mock-infected group（P〈0.01）. Taken together, S. pneumonia infection can induce CD103^＋DC, p DCs production, promotes IL-17A^＋CD4^＋Tcells and Tregs differentiation.

关 键 词：肺炎链球菌 感染 树突状细胞 CD4+T细胞 

分 类 号：R378.1[医药卫生—病原生物学]