肺炎链球菌诱导中性粒细胞胞外捕获网形成  

Streptococcus pneumoniae initiates the formation of neutrophil extracellular traps

作　　者：金春芳 黄益飞[1] 王子萌[1] 项云[1] 何於娟[1] 

机构地区：[1]重庆医科大学检验医学院,400016

出　　处：《免疫学杂志》2016年第4期310-314,共5页Immunological Journal

基　　金：国家自然科学基金(81373151);重庆市自然科学基金(2012jj A0035);重庆市教委科学技术研究项目(KJ130313)

摘　　要：目的初步探究肺炎链球菌诱导中性粒细胞胞外捕获网(NETs)形成的作用及调节机制,为进一步研究奠定基础。方法采用免疫磁珠法分选C57BL/6小鼠骨髓中性粒细胞,肺炎链球菌刺激中性粒细胞,分别采用脱氧核糖核酸酶(DNase)降解NETs和NADPH氧化酶抑制剂二苯基氯化碘盐(DPI)预处理中性粒细胞阻断活性氧簇(ROS)生成,免疫荧光染色观察NETs结构,检测游离DNA定量观察NETs的生成。结果肺炎链球菌刺激中性粒细胞释放NETs,DNase降解NETs结构,阻断ROS生成后NETs形成受抑。结论肺炎链球菌能够诱导NETs形成,DNase直接降解NETs或DPI抑制NETs形成可以成为调节NETs生成的有效途径。To investigate the effect of Streptococcus pneumoniae（S.pneumoniae） on the formation of neutrophilextracellular traps（NETs）, neutrophils were isolated from mouse bone marrow by magnetic activated cell sorting, andthen stimulated by S.pneumoniae for 180 minutes. NETs structure was observed by immunofluorescence stainingextracellular free DNA was detected by Sytox Green staining to quantify NETs. To further understand the roles ofdeoxyribonuclease（DNase） and reactive oxygen species（ROS） in NETs formation, DNase and NADPH oxidaseinhibitor diphenylene iodonium（DPI） which inhibits production of ROS were used. S.pn was found to be a potentinducer of NETs production and the formation of NETs was significantly decreased after being treated with DNase orDPI, which indicating that ROS was involved in the formation of NETs induced by S.pn. In conclusion, S.pn caninitiate the release of NETs. DNase and DPI could be inhibitors to balance formation of NETs.

关 键 词：肺炎链球菌 中性粒细胞 中性粒细胞胞外捕获网 活性氧簇 

分 类 号：R378.1[医药卫生—病原生物学]