NK-2受体参与小鼠断尾诱发的扣带回前部神经元Fos蛋白表达  

NK-2 Receptor Involved in Fos Protein Expression of Anterior Cingulate Gyrus Neurons Evoked by Amputation of the Tail Extremity of Mice

作　　者：张雅娟[1] 杨宇[2] 李娜然[3] 商丽宏[2] 吴孟霏 吴敏范[2] 

机构地区：[1]沈阳市第五人民医院神经内科,辽宁沈阳110023 [2]沈阳医学院基础医学院生理学教研室 [3]沈阳医学院基础医学院形态学实验室 [4]辽宁何氏医学院

出　　处：《沈阳医学院学报》2016年第2期68-69,77,共3页Journal of Shenyang Medical College

基　　金：沈阳市科技局计划项目(No.F13-220-9-45);辽宁省科技厅基金项目(No.L2015020391)

摘　　要：目的:探讨小鼠断尾能否改变扣带回前部(anterior cingulate gyrus,ACG)神经元Fos蛋白表达及NK-2受体在此变化中的作用。方法:采用免疫组织化学方法观察截断小鼠尾末端2.5 cm后0.5 h小鼠ACG神经元的Fos蛋白表达变化及蛛网膜下腔注射MEN-10.376(NK-2受体拮抗剂)对该变化的影响。结果:小鼠截断尾后0.5 h,ACG神经元的Fos蛋白表达显著增强;蛛网膜下腔注射MEN-10.376完全拮抗了截断尾诱发的ACG神经元的Fos蛋白表达显著增强,差异均有统计学意义。结论:中枢NK-2受体参与小鼠截断尾引起ACG神经元的Fos蛋白表达显著增强过程。Objective： To approach whether amputation of tail extremity of mice could evoke Fos protein expression change in ACG neurons and the role of NK-2 receptor in the change. Methods： Immunohistochemistry method was used to study Fos protein expression change in mice ACG neurons at 0. 5 h after amputation of the tail extremity 2. 5 cm,and effect of NK-2 receptor antagonist MEN-10. 376（ intrathecal injection） on the change. Results： Fos protein expression in mice ACG neurons significantly increased at 0. 5 h after the amputation. MEN-10. 376 completely antagonized the significant increase in Fos protein expression in mice ACG neurons after the amputation. There were significant differences. Conclusions： Amputation of the tail extremity can significantly increase the Fos protein expression of mice ACG neurons. Central NK-2 receptors are involved in the process.

关 键 词：幻肢痛 扣带回前部 FOS蛋白 MEN-10.376 NK-2受体 

分 类 号：R338[医药卫生—人体生理学] Q432[医药卫生—基础医学]