乙脑病毒SA14-14-2减毒株分枝杆菌检测分析  

Test for Mycobacteria on Attenuated Japanese Encephalitis Virus SA 14-14-2 Strain

作　　者：王红[1] 龙波[2] 杨筠[2] 吕冰凌[1] 唐溯[1] 潘海龙[1] 

机构地区：[1]成都生物制品研究所有限责任公司,成都610023 [2]四川省疾病预防控制中心

出　　处：《预防医学情报杂志》2016年第3期210-213,共4页Journal of Preventive Medicine Information

基　　金：国家科技支撑计划(2008BAI66B05);乙脑减毒活疫苗高技术产业化示范工程(发改办高技[2006]2352号)

摘　　要：目的对乙脑减毒活疫苗生产用毒种即乙脑病毒SA14-14-2减毒株的现行主种子批、工作种子批进行分枝杆菌检测,更好地控制外源因子对乙脑减毒活疫苗生产用毒种的污染。方法运用改良罗氏培养基培养法对乙脑病毒SA14-14-2减毒株的4个批号毒种样本进行分枝杆菌检测。结果 4个批号的乙脑病毒SA14-14-2减毒株毒种样本(主种子批,批号:9903;工作种子批,批号:JEV-W-7-20120701、JEV-W-7-20120702及JEV-W-7-20131201)的分枝杆菌培养结果无菌落生长。结论本次试验中4个批号的乙脑病毒SA14-14-2减毒株毒种样本分枝杆菌培养检测为阴性,说明乙脑毒种样本没有受到分枝杆菌污染,进而保障了乙脑减毒活疫苗的安全性。Objective To test attenuated Japanese encephalitis virus SA 14- 14- 2 strain（ Master seeds lot and working seeds lot） for mycobacteria in order to better control contamination of adventitious agents. Methods Improved Lowenstein-Jensen medium culture method was employed to test samples from four batches of attenuated Japanese encephalitis virus SA 14-14-2 seeds for mycobacteria. Results After cultivation of four batches of samples of attenuated Japanese encephalitis virus SA 14-14-2 seeds（ Master seeds lot,batch No. ： 9903; Working seeds lot,batch No. ： JEV-W-7-20120701,JEV-W-7-20120702 and JEV-W-7-20131201）,no mycobacteria colony was found. Conclusion The result of test for mycobacteria on four batches of samples of attenuated Japanese encephalitis virus SA 14-14- 2 seed is negative,indicating that attenuated Japanese encephalitis virus SA 14-14-2 seeds samples is not contaminated by mycobacteria,and therefore the safety of attenuated Japanese encephalitis virus SA 14-14-2 seeds could be proved.

关 键 词：乙脑减毒活疫苗 乙脑病毒SA14-14-2减毒株 改良罗氏培养基培养法 分枝杆菌检测 

分 类 号：R512.32[医药卫生—内科学]