通络救脑注射液对Aβ_(1-40)致脑微血管内皮细胞损伤的保护作用  

Protective Effects of Tongluo Jiunao Injection on Brain Microvascular Endothelial Cells Injured by β-amyloid 1-40

作　　者：高爱社[1,2] 杜欢[1] 范英昌[1] 王榭延 

机构地区：[1]天津中医药大学,天津300193 [2]河南中医药大学,河南郑州450008

出　　处：《中药新药与临床药理》2016年第2期157-160,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：高等学校博士学科点专项科研基金项目(20131210120004);天津市高等学校科技发展基金计划项目(20130213);河南省科技厅基础与前沿科研项目(142300410171)

摘　　要：目的探讨通络救脑注射液及其有效成分对脑微血管内皮细胞的保护作用及机制。方法建立β淀粉样蛋白(Aβ_(1-40))致人脑微血管内皮细胞(h BMECs)损伤模型,用通络救脑注射液及其有效单体成分京尼平苷、人参皂苷Rg1干预,采用CCK-8染色法测定细胞活性,检测条件培养液中乳酸脱氢酶(LDH)漏出量,Western blot法检测h BMECs高度糖基化终产物受体(RAGE)的表达。结果通络救脑注射液及其有效单体成分京尼平苷、人参皂苷Rg1可显著提高Aβ_(1-40)损伤的h BMECs活性,减少细胞损伤后LDH的漏出和h BMECs RAGE的表达,与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。结论通络救脑注射液及其有效单体成分京尼平苷、人参皂苷Rg1可提高Aβ_(1-40)损伤的h BMECs的活性,对其具有保护作用,其机制可能与减少血管内皮细胞RAGE的表达有关。Objective To investigate the protective effects and mechanism of Tongluo Jiunao（TLJN）injection and its active ingredients（including geniposde and ginsenoside Rg1）, on human brain microvascular endothelial cells（BMECs）injured by β- amyloid 1- 40（Aβ1-40）. Methods Human BMECs were pre- incubated by TLJN and momers geniposde and ginsenoside Rg1 for 2 h, and then injured by Aβ1-40. At different intervals, cell counting Kit- 8 was employed to assay the activities of BMECs, the leakage of lactate dehydrogenase in the culture of BMECs was examined,and the expression levels of receptor for advanced glycation end- products（RAGE）of BMECs was detected by Western blotting method. Results TLJN injection and its active ingredients（including geniposde and ginsenoside Rg1） could significantly improve the activities of human BMECs injured by Aβ1-40,decrease LDH leakage rate,and down- regulate the expression levels of RAGE（P〈0.05,P 〈0.01 compared with those of the model group）. Conclusion TLJN injection and its active ingredients（including geniposde and ginsenoside Rg1）has protective effects on the human BMECs injured by Aβ1-40,and its mechanism is related to the decrease of the expression of RAGE.

关 键 词：通络救脑注射液 脑微血管内皮细胞 高度糖基化终产物受体 Β淀粉样蛋白 

分 类 号：R285.5[医药卫生—中药学]