猪苓多糖通过调节HuR介导bcl-2表达诱导T24细胞凋亡  

Effect of Polyporus Polysaccharide on Inducing Apoptosis of T24 Cells by Regulating HuR- mediated bcl-2 Expression

作　　者：沈耿[1,2] 徐谦[2] 黄羽 曾星 

机构地区：[1]广州中医药大学第二附属医院,广东广州510120 [2]广州中医药大学基础医学院,广东广州510006

出　　处：《中药新药与临床药理》2016年第2期160-164,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81573769);广东省自然科学基金(2014A030313415);广东省中医药管理局立项课题(20151193);广州中医药大学基础医学院院长基金

摘　　要：目的研究猪苓多糖(polyporus polysaccharide,PPS)对膀胱癌T24细胞凋亡的作用及其机制。方法 T24细胞常规培养后加入不同剂量PPS,利用Hoechst染色、流式细胞术检测PPS对细胞凋亡的影响,利用q RT-PCR检测PPS对T24细胞bcl-2 m RNA表达水平及其稳定性的影响,通过Western Blot法检测PPS对T24细胞bcl-2蛋白、人抗原R(human antigen R,Hu R)蛋白的表达及对Hu R蛋白胞浆及胞核内分布的影响。结果与对照组比较,中、高剂量的PPS作用24 h后,T24细胞凋亡明显增多(P<0.05),bcl-2 m RNA稳定性降低(P<0.05),bcl-2蛋白及m RNA表达减少(P<0.05),总Hu R蛋白没有明显变化,但胞浆Hu R蛋白表达下降(P<0.05),胞核Hu R蛋白表达升高(P<0.05)。结论 PPS可诱导T24细胞凋亡,其作用可能与影响Hu R在细胞内定位,降低bcl-2 m RNA稳定性及减少bcl-2蛋白表达有关。Objective To study the effects of polyporus polysaccharide（PPS）on the apoptosis of human bladder cancer T24 cells and to explore the corresponding molecular mechanism. Methods The T24 cells in control group were normally treated and the cells in experimental groups were incubated with different dosages of PPS. The Hoechst staining and flow cytometry were used to analyze the influence of the drugs on cell apoptosis. The q RT- PCR was used to detect bcl- 2 mRNA levels of T24 cells. The level of human antigen R（HuR）protein in the cytoplasm and nuclei of T24 cells,and the bcl- 2 protein of T24 cells were detected by Western Blotting method. Results Compared with the control group,the cell apoptosis was obviously increased（P〈0.05）,the bcl- 2 m RNA stability and bcl- 2 mRNA and protein expression were decreased in the medium- and high- dose PPS groups（P〈0.05）,the total protein of Hu R was slightly changed, but the cytoplasm Hu R protein was down- regulated（P〈0.05） and the nucleus HuR protein was up- regulated（P〈0.05）. Conclusion PPS can induce the apoptosis of T24 cells,which is possibly related with the down- regulation of the cytoplasm HuR protein and with the decrease of bcl- 2 mRNA stability and bcl- 2 protein expression.

关 键 词：猪苓多糖 人抗原R 膀胱癌 T24细胞 凋亡 BCL-2 

分 类 号：R285.5[医药卫生—中药学]