甲氨蝶呤对银屑灵2号主要成分芍药苷体内药代动学参数的影响  

Effect of Methotrexate on Pharmacokinetics of Paeoniflorin,One Main Component of Yinxieling Recipe 2

作　　者：樊丹采 邓时贵 李杨[1] 赵瑞芝 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510405 [2]广东省中医药科学院,广东广州510000

出　　处：《中药新药与临床药理》2016年第2期231-233,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金团队项目(S2013030011515);广东省科技厅-广东省中医院联合专项项目(2011B032200009);广东省中医院项目(YK2013B1N11)

摘　　要：目的研究甲氨蝶呤(MTX)对中药复方银屑灵2号主要成分芍药苷药代动力学参数的影响,评价联合用药的合理性。方法将12只SD大鼠随机分为银屑灵2号组、联合用药组(MTX+银屑灵2号),单次口服给药后在不同时间点眼眶静脉采血,固相萃取(SPE)处理,采用HPLC测定血浆中芍药苷浓度,观察芍药苷在两组中的药代动力学参数的变化。结果与银屑灵2号组比较,联合用药组芍药苷AUC_(0-t)增加了29.50%,C_(max)增加了35.32%,T_(max)延迟了0.067 h,清除率降低了41.99%。结论 MTX与银屑灵2号联用可增加银屑灵2号中芍药苷的血药浓度,降低清除率,提高生物利用度。Objective To research the impact of Methotrexate（MTX）on the pharmacokinetics of paeoniflorin,which is the main component of Chinese medicine Yinxieling Recipe 2,and to evaluate the rationality of combination therapy.Methods Twelve Sprague- Dawley rats were divided randomly into Yinxieling Recipe 2 group and combined group（MTX combined with Yinxieling Recipe 2）,6 rats in each group. After single- dose oral administration,blood was sampled at different time points, and plasma samples were prepared by solid phase extraction method. Plasma paeoniflorin concentration was determined by HPLC method,and the changes of pharmacokinetics of paeoniflorin in the two groups were observed. Results Compared with Yinxieling Recipe 2 group, the AUC0-tof the combined group increased by 29.50 %,Cmax increased by 35.32 %,Tmax extended for 0.067 hours and the clearance rate decreased by41.99 %. Conclusion The drug concentration of paeoniflorin can be increased,the bioavailability is enhanced,and clearance rate is decreased when Yinxieling Recipe 2 is co- administrated with MTX.

关 键 词：芍药苷 银屑灵2号 甲氨蝶呤 联合用药 药物动力学 

分 类 号：R285.5[医药卫生—中药学]