骨髓间充质干细胞对糖尿病肾病大鼠肾小球紧密连接蛋白ZO-1表达的影响  

Effects of bone marrow mesenchymal stem cells on the expression of tight junction protein ZO - 1 in rats with diabetic nephropathy

作　　者：张袁[1] 高克瑜 戴春[2] 

机构地区：[1]徐州医学院研究生学院2013级,江苏徐州221004 [2]徐州医学院附属医院肾脏病科,江苏徐州221002

出　　处：《徐州医学院学报》2016年第2期114-118,共5页Acta Academiae Medicinae Xuzhou

摘　　要：目的探讨骨髓间充质干细胞（BMSCs）对糖尿病肾病（DN）大鼠肾小球ZO-1表达的影响。方法采用sD大鼠腹腔注射链脲佐菌素（STZ，55mg／kg）制备糖尿病模型，4周后尿蛋白〉30mg／d为DN模型成功（n=20），随机分为DN组（n=10）和BMSCs组（n=10），以另10只正常大鼠作为NC组（n=10）。BMSCs经体外培养、鉴定、5-溴脱氧尿嘧啶核苷（BrdU）标记后，于模型建立4周末尾静脉输注到BMSCs组大鼠体内。12周后处死大鼠，收集尿、血浆、肾组织，检测24h尿蛋白定量、血糖及血肌酐值，用免疫组化、Westernblot方法检测ZO-1的表达。结果与NC组比较，DN组和BMSCs组血糖、24h尿蛋白量、血肌酐显著增高（P〈0．05）；肾组织ZO-1蛋白表达减少（P〈0．05）。与DN组比较，BMSCs组24h尿蛋白量、血肌酐降低（P〈0．05），肾组织ZO-1表达增加（P〈0．05）。结论BMSCs对DN大鼠足细胞病变具有保护作用，其部分机制可能与上调ZO一1蛋白表达有关。Objective To investigate the effects of bone marrow mesenchymal stem ceils （BMSCs） on the expres- sion of ZO - 1 in the glomerulus of diabetic nephropathy （DN） rats. Methods SD rats were intraperitoneally injected with streptozotocin （STZ, 55 mg/kg） for establishment of a diabetic model. After 4 weeks, 20 rats with urinary protein 〉 30 mg/d were adopted in the current study and then randomly divided into a DN group and a BMSCs group （ n = 10 each）. Meanwhile, another ten healthy rats were selected as the control. BMSCs were cultured in vitro, identified and la- beled with 5 -bromo -2＇ -deoxyuridine （BrdU）, before transplanted via the tail vein in the BMSCs group at the end of the fourth week. The rats were sacrificed at the end of Week 12. Their urine, plasma and kidneys were collected, while 24 hours urinary protein content, blood glucose and creatinine were measured. The level of ZO - 1 was determined by Western blot. Results Compared with the control, both the DN and BMSCs groups presented remarkably increased lev- els of 24 hours urinary protein content, blood glucose, and serum creatinine （P 〈 0.05 ） but decreased amounts of ZO - 1 in the kidneys （P 〈 O. 05 ）. Compared with the DN group, the BMSCs group produced reduced amounts of 24 hours u- rinary protein content and serum creatinine （P 〈 0.05 ） but enhanced expression of ZO - 1 （ P 〈 0. 05 ）. Conclusion BMSCs may exert the protective effect on podocytes in diabetic nephropathy rats, which may be associated with the up - regulated expression of ZO - 1.

关 键 词：糖尿病肾病 骨髓间充质干细胞 足细胞损伤 ZO-1 

分 类 号：R587.3[医药卫生—内分泌]