肺炎克雷伯菌质粒pNDM-LJ的高通量测序分析  

High-throughput DNA sequencing of Klebsiella pneumoniae plasmid pNDM-LJ

作　　者：杨羚[1] 唐海玲[2] 陈定强[1] 裘宇容[3] 

机构地区：[1]广州医科大学附属第一医院检验科,510120 [2]广州医科大学附属第一医院转化医学实验室,510120 [3]南方医科大学南方医院检验科,广州市510515

出　　处：《实用医学杂志》2016年第5期706-709,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81201341)

摘　　要：目的:通过对肺炎克雷伯菌质粒pNDM-LJ的高通量测序分析,研究其与肺炎克雷伯菌耐药的相关性。方法:利用Illumina Miseq高通量测序平台进行测序,然后用Edena软件拼接测序数据,再利用RAST网上工具将拼接得到的contigs进行注释,并用BLAST网上工具进行分析。结果:pNDM-LJ为54 kb大小的环状质粒,GC含量约为49%,预测编码52个功能基因,在不相容群分类中属于Inc X3群质粒。该质粒与已知的Inc X质粒序列有较高的相似性,blaNDM-1基因所在的基因环境比较复杂,推测其是由多个转座事件共同构建的。在pNDM-LJ中,blaNDM-1基因的5′端和3′端分别与ISAba125和IS26两种插入序列相连,形成大小约为10.8 kb的转座子样结构。结论:pNDM-LJ质粒携带碳青霉烯类耐药基因blaNDM-1,可能对我国的广泛传播blaNDM-1基因发挥重要作用。Objective To investigate the relationship between the resistance of the Klebsiella pneumoniae and the Klebsiella pneumoniae plasmid p NDM-LJ carrying bla NDM-1 by high-throughput DNA sequencing.Methods High-throughput DNA sequencing was carried out by the Illumina Miseq platform, and sequencing data were assembled by Edena software. Contigs were annotated by the RAST server and analyzed by the BLAST server. Results The plasmid p NDM-LJ was 54-kb in size with a GC content of 49%. The plasmid encoded 52 putative functional genes and belonged to the Inc X3 group in incompatible classifications. Analysis of the plasmid sequence revealed high similarity with other Inc X3 plasmids. The bla NDM-1 gene was located in a complicated gene environment possibly constructed by several transposition events. The 5′ and 3′ ends of the bla NDM-1 gene were adjacent to the ISAba125 and IS 26 respectively, forming a 10.8-kb transposon-like structure. Conclusion The plasmid p NDM-LJ carried the bla NDM-1 gene being resistant to carbapenems and played a possibly important role in transmission of bla NDM-1 in China.

关 键 词：肺炎克雷伯菌 p NDM-LJ blaNDM-1基因 碳青霉烯酶 

分 类 号：R446.5[医药卫生—诊断学]