地塞米松对大鼠肝细胞BRL-3A增殖和凋亡的影响  

Effect of dexamethasone on the proliferation and apoptosis of rat liver cell line BRL-3A

作　　者：常翠芳[1,2,3] 赵卫明[1,2,3] 杨婧[1,2,3] 李晓芳[1,2,3] 陈莎莎[1,2,3] 王改平[1,2,3] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省-科技部共建细胞分化国家重点实验室培育基地和河南省生物工程重点实验室,河南新乡453007 [3]河南省生物工程药物开发实验室,河南新乡453007

出　　处：《解剖学报》2016年第2期197-202,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(31201093);河南师范大学博士科研启动费支持项目(11134);河南省自然科学基金(112300413204)

摘　　要：目的探讨地塞米松对体外培养大鼠肝细胞BRL-3A增殖和凋亡的影响。方法用不同浓度的地塞米松处理BRL-3A细胞,于12、24、48、72、120 h后用MTT检测地塞米松对BRL-3A细胞活性的影响,同时分别用Annexin V-FITC双染法、PI单染色法、实时定量-聚合酶链反应(Real-time PCR)等方法检测地塞米松对BRL-3A细胞周期和凋亡的影响。结果 MTT检测表明,地塞米松能够抑制BRL-3A细胞的活性;Annexin V-FITC双染法分析显示,地塞米松具有显著促进BRL-3A细胞凋亡的效果;PI单染色法检测细胞周期分布情况表明,地塞米松处理后的细胞与对照组相比增殖能力减弱;用Real-time PCR检测促细胞凋亡基因Caspase-3、Caspase-8、Caspase-9和促增殖基因Ccnd1和Jun的mRNA表达情况,结果显示,用地塞米松处理后的细胞中Caspase-3、Caspase-8和Caspase-9均表达上调,而Ccnd1和Jun均表达下调,说明地塞米松能促进BRL-3A细胞的凋亡。结论地塞米松可以促进BRL-3A细胞的凋亡,并抑制其增殖。Objective To explore the effect of dexamethasone on cell proliferation and apoptosis in the rat liver cell line BRL-3A in vitro. Methods BRL-3A cells were treated with different concentrations of dexamethasone,and MTT method was used to observe the effect of dexamethasone on cell activity at 12,24,48,72 and 120 hours after treatment.Annexin V-FITC staining and propidium iodide（ PI） staining were used to detect the effect of cell apoptosis and cell cycle.Real-time PCR was used to evaluate the changes in the expression of related genes. Results The result of MTT assays revealed that dexamethasone inhibited the proliferation of BRL-3A cells in a dose-dependent manner. Annexin V-FITC staining showed that dexamethasone significantly induced the apoptosis of BRL-3A. PI staining indicated that the ability of proliferation decreased in the cells treated with dexamethasone. Real-time PCR analysis showed that pro-apoptosis genes Caspase-3,Caspase-8 and Caspase-9 were up-regulated,while pro-proliferation genes Ccnd1 and Jun were down-regulated.Conclusion Dexamethasone may inhibit the proliferation of BRL-3A cell line and induce its apoptosis by up-regulating Caspase-3,Caspase-8 and Caspase-9 and down-regulating the expression of Ccnd1 and Jun.

关 键 词：地塞米松 BRL-3A细胞 流式细胞术 实时定量-聚合酶链反应 大鼠 

分 类 号：Q28[生物学—细胞生物学]