磷酸化信号转导和转录激活因子3(Y705)在子宫腺肌症中的表达及其临床意义  

Expression of phosphorylated-signal transduction and activator of transcription 3(Y705) in adenomyosis and their clinical significances

作　　者：王静[1] 杨阳[1] 杨宁[2] 邓晓惠[1] 杨兴升[3] 晁岚[1] 

机构地区：[1]山东大学齐鲁医院不孕不育诊疗中心,济南250012 [2]山东大学齐鲁医院不孕病理科,济南250012 [3]山东大学齐鲁医院妇产科,济南250012

出　　处：《解剖学报》2016年第2期261-267,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(30901603;81370711;81571511);山东省科技攻关项目(2010GSF10814);山东大学齐鲁医院科研基金;山东大学基本科研业务费专项资金(2015QLMS24;2015QLQN50)

摘　　要：目的通过检测磷酸化信号转导和转录激活因子3(p-STAT3)(Y705)和血管内皮生长因子(VEGF)在正常子宫内膜与子宫腺肌症在位内膜中的定位与表达,探讨p-STAT3(Y705)和VEGF在子宫腺肌症中发生发展中的作用。方法免疫组织化学SP法检测14例正常子宫内膜和14例子宫腺肌症在位内膜中p-STAT3(Y705)和VEGF的定位和表达情况;Real-time PCR检测正常子宫内膜与子宫腺肌症在位内膜组织中STAT3 mRNA表达情况;Western blotting检测正常子宫内膜与子宫腺肌症在位内膜组织中p-STAT3(Y705)、STAT3和VEGF蛋白的表达水平。结果 p-STAT3(Y705)主要在正常子宫内膜和子宫腺肌症在位内膜腺上皮细胞核中表达,子宫腺肌症在位内膜蛋白表达高于正常子宫内膜(P<0.05),正常子宫内膜和子宫腺肌症在位内膜相应的增殖期和分泌期p-STAT3(Y705)的表达无显著性差异(P>0.05)。正常子宫内膜和子宫腺肌症在位内膜中,STAT3在mRNA和蛋白表达水平差异均无显著性(P>0.05)。VEGF主要表达在腺上皮细胞中,子宫腺肌症在位内膜蛋白表达高于正常子宫内膜(P<0.05),正常子宫内膜和子宫腺肌症在位内膜相应的增殖期和分泌期VEGF的表达差异无显著性(P>0.05)。结论 p-STAT3(Y705)促进血管生成对子宫腺肌症的发生发展有一定的作用。Objective To investigate the role of phosphorylated-signal transduction and activator of transcription 3（ p-STAT3）（ Y705） and vascular endothelial growth factor（ VEGF） in the development of adenomyosis by detecting the expression of p-STAT3（ Y705） and VEGF in normal endometrium and eutopic endometrium of adenomyosis patients.Methods The localization and expression of p-STAT3（ Y705） and VEGF in 14 cases of normal endometrium and 14 cases of eutopic endometrium of adenomyosis patients were detected by immunohistochemical SP method. Real-time PCR was performed to understand the expression of signal transduction and activator of transcription 3（ STAT3） mRNA in normal endometrium and eutopic endometrium of adenomyosis patients. The protein levels of p-STAT3（ Y705）,STAT3 and VEGF in normal endometrium and eutopic endometrium of adenomyosis patients were detected by Western blotting. Results In the normal endometrium and eutopic endometrium of adenomyosis,p-STAT3（ Y705） was mainly located in the nucleus of the glandular epithelium. p-STAT3（ Y705） had higher expression in adenomyosis groups than that in control（ P〈 0. 05）.There was no significant difference in the expression of p-STAT3（ Y705） in the proliferative phase and secretory phase ofeach group（ P〉 0. 05）. In the normal endometrium and eutopic endometrium of adenomyosis,there was no significant difference in the expression of STAT3 mRNA and protein levels（ P〉0. 05）. VEGF immunostaining mainly localized in the the glandular epithelial cells. In ectopic endometrium of adenomyosis,the expression of VEGF was significantly higher than the controls（ P〈 0. 05）. There was no significant difference in the expression of VEGF in the proliferative phase and secretory phase of each group（ P〉 0. 05）. Conclusion p-STAT3（ Y705） may play a role via promoting angiogenesis in the development of the adenomyosis.

关 键 词：子宫内膜 子宫腺肌症 磷酸化信号转导和转录激活因子3(Y705) 信号转导和转录激活因子3 血管内皮生长因子 免疫组织化学 实时定量聚合酶链反应 人 

分 类 号：R711[医药卫生—妇产科学]