慢病毒介导的胶质瘤模型在教学中的应用  

Application of slow virus mediated glioma model in teaching

作　　者：宋大勇[1] 李志强[1] 权哲[1] 赵军[1] 张宁[1] 许大远[1] 

机构地区：[1]上海交通大学附属第六人民医院南院,上海201499

出　　处：《解剖学报》2016年第2期281-283,共3页Acta Anatomica Sinica

摘　　要：目的比较移植性肿瘤动物模型与慢病毒介导的胶质瘤模型在教学中的应用效果。方法选取50名临床8年制5年级学生,根据不同的实验操作,将学生随机分为移植性肿瘤动物模型组(n=25)以及慢病毒介导的胶质瘤模型组(n=25)。移植性肿瘤动物模型组以大鼠胶质瘤细胞系9L脑纹状体注射Wistar大鼠模型进行实验操作;而慢病毒介导的胶质瘤模型组则以慢病毒载体介导Ras的表达以及Akt的激活,注射胶质纤维酸性蛋白(GFAP)-Cre基因小鼠进行实验操作。教学结束后比较两组的教学反馈和教学成果。结果与移植性肿瘤动物模型组相比,慢病毒介导的胶质瘤模型组成员对该模型的教学反馈评价更高,该模型的重复性更好,使学生对肿瘤分子水平的认识更深入,差异具有统计学意义(P<0.05)。结论慢病毒介导的胶质瘤模型在教学中应用具有可行性,不仅提供了一个较新的模型,也使得学生能够从分子水平认识肿瘤,对于肿瘤的理解更为深入。Objective To compare the application effect in teaching between portability tumor animal models and slow virus mediated glioma model. Methods Fifty students of grade six majoring in eight-year clinical oncology were randomly divided into two teaching groups,25 students per group. Two groups performed different experimental operations：portability tumor animal model group injected rat glioma cell line 9L brain striatum into Wistar rat model,while slow virus mediated glioma model group injected GFAP-Cre mice by Ras expression and Akt activation mediaed by slow virus carrier.The teaching feedback and teaching achievements of two groups after teaching were compared. Results Compared with portability tumor animal model group,the slow virus mediated glioma model group members showed higher feedback of teaching evaluation. The repeatability of the second model was better, which helped students make more sound understanding of tumor at the molecular level. There was a statistically significant difference（ P〈 0. 05）. Conclusion Slow virus mediated glioma model is feasible for application in the teaching,which not only provides a newer model,also makes the students know the tumor at the molecular level,and get thoroughly understanding of the tumor.

关 键 词：慢病毒介导的胶质瘤模型 转基因型脑胶质瘤模型 移植脑胶质瘤模型 教学 学生 

分 类 号：G642[文化科学—高等教育学]