超高效液相色谱串联质谱法快速测定尼扎替丁在人体血浆中的含量  

Rapid determination of nizatidine in human plasma by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：段虹飞[1] 赵宁民[1] 李巧艳[1] 马爱玲[1] 王漪檬[1] 赵红卫[1] 张伟[1] 秦玉花[1] 

机构地区：[1]河南省人民医院药学部,河南郑州450000

出　　处：《中国医院药学杂志》2016年第5期355-358,共4页Chinese Journal of Hospital Pharmacy

基　　金：河南省医学科技攻关项目(编号:201303174)

摘　　要：目的：建立超高效液相色谱串联质谱法测定人体血浆中尼扎替丁的含量。方法：血浆以甲醇直接沉淀蛋白,上清液用于目标物的检测;色谱柱为Waters ACQUITY UPLC BEH C18;流动相为5 mmol·L^-1醋酸铵水溶液-甲醇（65∶35,V/V）;流速：0.20 mL·min^-1;进样量：3μL;柱温：35℃;样品室温度：6℃;采用电喷雾正离子模式电离,尼扎替丁和内标雷尼替丁的监测离子分别为（m/z）332.2→155.0,（m/z）315.2→130.0。结果：尼扎替丁的线性范围为10-3 000 ng·mL^-1,平均回收率为93.2%-101.1%,批内、批间RSD均小于15%。结论：本研究建立的分析方法快速、准确、灵敏、简便,适用于尼扎替丁人体药动学方面的研究。OBJECTIVE To establish an ultra-high performance liquid chromatography-tandem mass spectrometry（ UPLC-MS /MS） method for determination of nizatidine in human plasma. METHODS Plasma samples were deproteinized by methanol and separated on a Waters ACQUITY UPLC BEH C18 column. Column temperature was set at 35 ℃,5 mmol·L^- 1ammonium acetate- methanol（ 65∶ 35,V/V） as mobile phase,at a flow rate of 0. 20 mL·min^- 1. Sample room temperature was set at 6 ℃ and injection volume at3 μL. Electorspray source and positive ion scan were used,nizatidine and internal standard were monitored at（ m / z） 332. 2→155. 0and（ m / z） 315. 2→130. 0,respectively. RESULTS Calibration curve of nizatidine was linear in range of 10-3 000 ng·mL^- 1. Mean recoveries were from 92. 2% to 97. 9% at spiked levels of 25. 18,503. 50 and 2 517. 50 ng·mL^- 1. Relative standard deviations（ RSDs） of intra-run and inter-run were both less than 15%. CONCLUSION The method is rapid,sensitive,simple and accurate for determination of nizatidine in human plasma and suitable for pharmacokinetic study.

关 键 词：超高效液相色谱串联质谱法 尼扎替丁 血药浓度 

分 类 号：R969[医药卫生—药理学]