薄层色谱-显微红外光谱(TLC-MicroIR)联用技术检测减肥保健食品中非法添加盐酸芬氟拉明  

Determination of fenfluramine hydrochloride added illegally into anti-obesity and healthcare food by TLC-Micro IR method

作　　者：许凤[1] 冯钰[1] 付双[1] 李莉[1] 

机构地区：[1]黑龙江省齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《中国医院药学杂志》2016年第5期358-361,共4页Chinese Journal of Hospital Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目(编号:12521650);齐齐哈尔医学院院内科研基金项目资助(编号:QY2016Z-22)

摘　　要：目的:建立减肥保健食品中非法添加盐酸芬氟拉明的定性检测方法。方法:供试品用甲醇超声提取,以硅胶GF254铝合金薄层板(5 cm×10 cm)为固定相,以三氧甲烷-甲醇-氨水(11∶1∶0.12)为展开剂,在紫外灯254 nm下检视,用薄层色谱法(TLC)快速初筛后,再联用显微红外光谱法(Micro IR)对其进行定性检测。结果:在检查下的9批供试品中有2批含有盐酸芬氟拉明。结论:所建立的方法专属性强、灵敏度高、重现性好,可以作为该类保健食品非法添加盐酸芬氟拉明的快速检测方法。OBJECTIVE To establish an qualitative method for fenfluramine hydrochloride illegally added into anti-obesity and healthcare food. METHODS Samples were extracted with methanol. Stationary phase was silica gel GF254 aluminum alloy plates（ 5cm × 10 cm） and developing solvent was chloroform-methanol-ammonia（ 11∶ 1∶ 0. 12） to observe the samples under 254 nm UV lamp for primary screening,then micro infrared spectroscopy was combined for qualitative detection. RESULTS Two of nine samples contained fenfluramine hydrochloride. CONCLUSION The method has strong specificity,high sensitivity,fast and good reproducibility,so it can be used to detect fenfluramine hydrochloride illegally added into anti-obesity and healthcare food.

关 键 词：减肥保健食品 盐酸芬氟拉明 薄层色谱法 显微红外光谱法 

分 类 号：R927.2[医药卫生—药学]