抗生素对溶藻弧菌SXT/R391元件转移频率的影响  

Effects of antibiotics on the transfer frequency of SXT/R391 element of Vibrio alginolyticus

作　　者：何香燕[1,2] 罗鹏[1] 高沿[1,2] 胡超群[1] 韦露[1,2] 刘秋婷 

机构地区：[1]中国科学院南海海洋研究所,中科院热带海洋生物资源与生态重点实验室,广东省应用海洋生物学重点实验室,广东广州510301 [2]中国科学院大学,北京100049

出　　处：《微生物学报》2016年第4期643-650,共8页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31370149);中国科学院知识创新工程项目(KSCX2-EW-G-12B)~~

摘　　要：【目的】研究萘啶酸、诺氟沙星、卡那霉素3种抗生素对溶藻弧菌(Vibrio alginolyticus)SXT/R391元件ICEVal A056-1转移频率的影响。【方法】利用PCR检测溶藻弧菌A056中ICEVal A056-1的自我剪切、转移潜力。通过溶藻弧菌A056与大肠杆菌菌株VB111的接合实验,研究溶藻弧菌分别在含不同浓度萘啶酸、诺氟沙星、卡那霉素的LB培养基中培养15 min或30 min后,ICEVal A056-1转移频率的变化规律。【结果】溶藻弧菌A056细胞中有环状形式的ICEVal A056-1分子存在,具有水平转移潜力;溶藻弧菌A056在含40μg/m L萘啶酸的LB中培养30 min后,ICEVal A056-1转移频率是对照组的19.59倍;在含50μg/m L诺氟沙星的LB中培养15 min后,ICEVal A056-1转移频率是对照组的31.25倍;在含不同浓度卡那霉素的LB中培养30 min后,ICEVal A056-1转移频率与对照组没有显著差别。【结论】部分抗生素的使用可以明显促进溶藻弧菌ICEVal A056-1向大肠杆菌的转移,因此海洋环境中抗生素的滥用及随意排放很可能加剧ICEs(integrating conjugative elements)从溶藻弧菌到其他细菌的传播。[Objective] We studied the effects of nalidixic acid,norfloxacin and kanamycin on the transfer frequency of SXT/R391 element ICEVal A056-1 in Vibrio alginolyticus.[Methods] The circular ICEVal A056-1 in V.alginolyticus A056 was detected by PCR.Conjugation experiments were conducted between V.alginolyticus A056 and Escherichia coli VB111 to explore the frequency variation of the integrating conjugative elements transfer after donor strain A056 was cultured in Luria Broth containing nalidixic acid or norfloxacin or kanamycin in different concentrations for 15 min or 30 min.[Results] Circular ICEVal A056-1 was detected in V.alginolyticus A056,indicating that ICEVal A056-1 had the potential to transfer.Treatment with 40 μg/m L nalidixic acid for 30 min increased the transfer frequency of ICEVal A056-1 to19.59 folds.Treatment with 50 μg/m L norfloxacin for 15 min increased the transfer frequency of ICEVal A056-1 to 31.25 folds.The transfer frequency of ICEVal A056-1 had no significant changes under treatment with different concentrations of kanamycin for 30 min.[Conclusion] This study indicates that some antibiotics can obviously increase the transfer frequency of ICEVal A056-1,and that antibiotics abuse and arbitrarily discharge might intensify dissemination of integrating conjugative elements from V.alginolyticus to other bacteria.

关 键 词：溶藻弧菌 SXT/R391元件 转移频率 抗生素 

分 类 号：Q78[生物学—分子生物学]