基于16S rRNA和RubisCO基因对嗜酸硫杆菌的系统发育及多样性  

Phylogenetic and diversity analysis of Acidithiobacillus spp.based on 16S rRNA and RubisCO genes homologues

作　　者：刘敏瑞 蔺朋武 齐兴娥 倪永清[1] 

机构地区：[1]石河子大学食品学院,新疆石河子832000 [2]石河子大学生命科学学院,新疆石河子832000

出　　处：《微生物学报》2016年第4期664-679,共16页Acta Microbiologica Sinica

基　　金：国家自然基金(31260001;C010101)~~

摘　　要：【目的】明确不同地理来源的Acidithiobacillus spp.种群是否表现出显著的地域性和异域物种形成;为了解微生物谱系地理、多样性维持机制和微生物分子地理学提供基础数据。【方法】采用16S r RNA基因、核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubis CO)功能基因序列同源性分析构建相应的系统发育树,分析Acidithiobacillus spp.种群的遗传多样性。【结果】从3个不同的地域分离到35株菌聚为5大类群,其中菌株YNTR4-15可能是铁氧化细菌(Leptspirillum ferrooxidans),菌株HBDY3-3被鉴定为另一铁氧化细菌(Leptospirillum ferrodiazotrophum);有4株可能是Acidithiobacillus ferrivorans;6株是Acidithiobacillus ferridurans,其余菌株均被鉴定为Acidithiobacilus ferrooxidan。对26株代表性菌株的Rubis CO I型cbb L基因和II型cbb M基因的分析,发现19株菌具有双拷贝cbb L基因,分别为cbb L1和cbb L2基因;7株菌只检测到了cbb L1。cbb L1和cbb L2基因都有3个序列型;而cbb M基因是单拷贝。Rubis CO基因的密码子偏爱性不强。【结论】分离自3个地域的菌株16S r RNA/Rubis CO基因存在序列差异,Acidithiobacillus spp.种群存在显著的遗传多样性。嗜酸硫杆菌分离菌株基于16S r RNA基因的系统发育树和Rubis CO基因的发育树不一致。[Objective] The purpose of the study was to reveal geographic region-related Acidithiobacillus spp.distribution and allopatric speciation.Phylogenetic and diversity analysis was done to expand our knowledge on microbial phylogeography,diversity-maintaining mechanisms and molecular biogeography.[Methods] We amplified16 S r RNA gene and Rubis CO genes to construct corresponding phylogenetic trees based on the sequence homology and analyzed genetic diversity of Acidithiobacillus spp..[Results] Thirty-five strains were isolated from three different regions in China（Yunnan,Hubei,Xinjiang）.The whole isolates were classified into five groups.Four strains were identified as A.ferrivorans,six as A.ferridurans,YNTR4-15 Leptspirillum ferrooxidans and HBDY3-31 as Leptospirillum ferrodiazotrophum.The remaining strains were identified as A.ferrooxidans.Analysis of cbb L and cbb M genes sequences of representative 26 strains indicated that cbb L gene of 19 were two copies（cbb L1 and cbb L2）and 7 possessed only cbb L1.cbb M gene was single copy.In nucleotide-based trees,cbb L1 gene sequences of strains were separated into three sequence types,and the cbb L2 was similar to cbb L1 with three types.Codon bias of Rubis CO genes was not obvious in Acidithiobacillus spp..[Conclusion] Strains isolated from three different regions in China indicated a great genetic diversity in Acidithiobacillus spp.and their 16 S r RNA/Rubis CO genes sequence was of significant difference.Phylogenetic tree based on 16 S r RNA genes and Rubis CO genes was different in Acidithiobacillus spp..

关 键 词：ACIDITHIOBACILLUS spp. 16S RRNA基因 RUBISCO基因 系统发育 遗传多样性 

分 类 号：Q933[生物学—微生物学]