人参皂苷Re对H9c2心肌细胞CYP450酶的影响  

Effect of ginsenoside Re on cytochrome P450 in H9c2 cells

作　　者：马增春[1] 肖勇[1] 赵佳伟[1,2] 王宇光[1] 谭洪玲[1] 梁乾德[1] 汤响林[1] 肖成荣[1] 杨凌[3] 高月[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]安徽医科大学研究生学院,安徽合肥230032 [3]南京军区杭州疗养院海勤疗养区,浙江杭州310002

出　　处：《中国药理学通报》2016年第4期494-498,共5页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(No2012CB518402);国家自然科学基金资助项目(No81274127);国家科技重大专项(No2014ZX09J14104-01A)

摘　　要：目的观察人参皂苷Re对H9c2心肌细胞色素P450酶的影响,旨在发现人参皂苷Re对心肌细胞作用的分子机制。方法实验分对照组、人参皂苷Re各剂量组(1、5、10、50、100μmol·L^(-1))处理组,人参皂苷Re作用6、24、36、48及60 h后提取RNA或蛋白进行测定。利用实时定量PCR法检测H9c2心肌细胞CYP2C11、CYP2J3、CYP4A1、CYP4A3、CYP4F4及ANP mRNA的表达;采用Western blot法检测心肌细胞CYP4A1和CYP2J3蛋白的表达。结果人参皂苷Re明显上调心肌细胞CYP2C11、CYP2J3 mRNA的表达至正常对照的1.6、1.8倍,明显下调CYP4A1、CYP4A3及CYP4F4 mRNA的表达至正常对照的0.4、0.15、0.3倍。人参皂苷Re明显上调ANP基因表达水平至正常对照的3.2倍。随着药物浓度的增加,人参皂苷Re对CYP4A1蛋白表达产生明显的下调作用,而对CYP2J3蛋白产生明显的上调作用。结论人参皂苷Re可影响心肌细胞的CYP450酶和ANP基因的表达。Aim To observe the cytochrome 450 effect of ginsenoside Re on H9c2 cells,in order to clarify the molecular mechanism of ginsenoside Re. Methods H9c2 cells were separately treated with ginsenoside Re for 1,5,10,50,100 μmol·L^(-1) or 6,24,36,48,60 h. CYP2C11,2J3,4A1,4A3,4F4 and ANP mRNA expressions were analyzed by Real time PCR,and CYP4A1,2J3 protein expressions were detected by Western blot. Results Compared with control group,ginsenoside Re could effectively upregulate CYP2C11,CYP2J3,ANP mRNA expression to 1. 6,1. 8,3. 2fold,and downregulate CYP4A1,CYP4A3,CYP4F4 mRNA expression to 0. 4,0. 15,0. 3 fold. Ginsenoside Re could decrease CYP4A1 protein expression in a concentration-dependent manner,while ginsenoside Re could increase CYP2J3 protein expression in a concentration-dependent manner. Conclusion Ginsenoside could regulate CYP450 enzyme and change ANP gene expression,which might be the molecular mechanism of ginsenoside Re.

关 键 词：人参皂苷RE 心肌细胞 利钠肽 细胞色素P450酶 CYP2J3 CYP4A1 

分 类 号：R284.1[医药卫生—中药学] R322.11[医药卫生—中医学]