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作 者:王国伟[1]
出 处:《广州体育学院学报》2016年第2期96-99,共4页Journal of Guangzhou Sport University
基 金:河南省高等学校重点科研项目计划(15A890003)
摘 要:目的:观察不同频率振动训练对大鼠有氧工作能力的影响,探讨振动训练增强大鼠骨骼肌有氧工作能力的分子生物学机制。方法:4周龄雄性SD大鼠40只适应性饲养1周后,随机分为安静对照组(C,n=10)、低频率振动训练组(L,15Hz,n=10)、中频率振动训练组(M,25Hz,n=10)和高频率振动训练组(H,35Hz,n=10),经过8周振动训练后,测试各组大鼠跑台运动时间,检测各组大鼠腓肠肌VEGF、KDR、Ang-1及b FGF蛋白表达水平。结果:L、M两组大鼠的跑台运动时间较C组显著增加(P<0.01),且M组大鼠运动持续时间最长;各振动训练组大鼠腓肠肌VEGF、KDR、Ang-1及b FGF蛋白表达水平均显著高于安静对照组(P<0.05),M组大鼠腓肠肌各蛋白表达水平显著高于其它各组。结论:不同频率振动训练均通过提高大鼠腓肠肌VEGF、KDR、Ang-1及b FGF蛋白表达增强了骨骼肌的有氧工作能力,中等频率振动训练效果最为显著。Objective: The purpose of this study is to discuss the mechanism of vibration training in improving muscle aerobic capacity from the biomechanics. Methods: 40 male rats of 4 weeks old were taken for experiment. The rats were divided into control group( C,n = 10),15 Hz training group( L,15 Hz,n = 10),25 Hz training group( M,25 Hz,n = 10),35 Hz training group( H,35 Hz,n = 10). At the end of the experiment,the aerobic capacity changes of four groups of rats were compared and the gastrocnemius VEGF、KDR、Ang- 1 and b FGF protein expression level of each group of rat were detected. Results: The aerobic capacity of M group is increased very significantly( P〈 0. 01); The VEGF、KDR、Ang- 1 and b FGF protein expression of gastrocnemius tissue in C group were significantly lower than that of H group( P〈 0. 01),The VEGF、KDR、Ang- 1 and b FGF protein expression of gastrocnemius tissue in M group were significantly higher than that of all groups( P〈 0. 05). Conclusion: The mechanism of improving muscle aerobic capacity by vibration trainning is to improve VEGF、KDR、Ang- 1 and b FGF protein expression of gastrocnemius.
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