上海市男男性行为者中感染HIV-1的CRF01_AE毒株传播簇分析  

HIV-1 transmission clusters among men who have sex with men in Shanghai

作　　者：林怡[1] 薛以乐[1] 王绪琴[1] 周磊明[1] 王盈[1] 沈方伟[1] 程华[1] 庄鸣华[1] 钟平[1] 

机构地区：[1]上海市疾病预防控制中心,上海200336

出　　处：《中国艾滋病性病》2016年第3期165-169,共5页Chinese Journal of Aids & STD

基　　金：国家"十二五"传染病科技重大专项(2012ZX10001-002);病毒学国家重点实验室开放研究基金项目(2015IOV004)~~

摘　　要：目的了解上海地区男男性行为者(MSM)中,感染艾滋病病毒1型(HIV-1)的CRF01_AE重组型的流行情况、成簇特性,为HIV防控工作提供科学数据。方法从2009-2013年每年新诊断的MSM HIV-1感染者血浆样本中提取RNA,并对HIV-1 pol基因片段进行反转录和巢式PCR扩增、DNA测序和亚型分析。对CRF01_AE重组型序列进行系统进化学和社会人口学信息分析。结果 1262名MSM感染者中,CRF01_AE重组型感染782人(62.0%),其序列在系统进化树上形成6个流行簇。使用不同参数界定传播簇并进行比较,选择以Bootstrap≥90%及基因距离≤0.03作为传播簇的界定标准,形成112个传播簇,其中由10条及以上序列形成的传播簇有5个;年龄、文化程度、婚姻状况、性病史和CD4+T淋巴细胞计数与样本的成簇性有统计学关联。结论 CRF01_AE重组型毒株是上海市MSM HIV-1感染者中的优势传播株,具有较强的成簇性,并且与相关的社会人口学和流行病学背景相关。Objective To characterize the propagation and clustering of HIV-1CRF01_AE strain among men who have sex with men（MSM）in Shanghai.Methods Viral RNA was extracted from plasma samples derived from newly diagnosed HIV-1-infected MSM from 2009 to 2013.HIV-1pol gene segments were amplified using RT-PCR and nested PCR,sequenced and phylogenetically analyzed.Bioinformatics analysis was applied on the formation of clustering for the identified CRF01_AE strains.Results 782 individuals were identified as CRF01_AE based on pol gene phylogenetic tree and constructed six epidemic clusters.In the context of bootstrap≥90% and gene distance≤0.03,112 transmission clusters were found and 5of them consisted of 10 or more sequences.Age,education,marriage status,STD history and CD4＋T cell count were statistically related to the sequences clustering.ConclusionAs the dominating strain among HIV-1-infected MSM in Shanghai,CRF01_AE is inclined to form clusters,and relating to demographic and epidemiological characteristics.

关 键 词：艾滋病病毒1型 男男性行为者 CRF01_AE 传播簇 

分 类 号：R373.9[医药卫生—病原生物学]