1,25-(OH)_2维生素D3对再生障碍性贫血患者T细胞亚群的影响  

Effects of 1,25-(OH)_2 D3 on T cell subsets in patients with aplastic anemia

作　　者：陆静涛[1] 杨建和[1] 章艳[1] 何金媛[1] 陈涛[1] 卢绪章[1] 

机构地区：[1]南京医科大学附属常州第二人民医院血液科,江苏常州213000

出　　处：《临床血液学杂志》2016年第2期207-208,212,共3页Journal of Clinical Hematology

摘　　要：目的:研究再生障碍性贫血(AA)患者外周血中的T细胞亚群变化与血清中1,25-(OH)_2维生素D3水平的关系。方法:利用流式细胞仪检测15例AA患者和25例健康对照者外周血T细胞亚群的变化,并用ELISA法检测AA患者和健康对照者血清中1,25-(OH)_2维生素D3的水平。结果:AA患者血清中1,25-(OH)_2维生素D3水平明显低于健康对照者[(35.5±11.8)pmol/L∶(51.8±12.6)pmol/L,P<0.05]。与健康对照者比较,AA患者外周血中CD4在淋巴细胞中比率无明显变化[(31.8±4.7)%∶(33.6±4.2)%,P=0.217],但CD8在淋巴细胞中比率显著升高[(31.5±5.2)%∶(24.8±3.8)%,P<0.01],故CD4/CD8比值降低(P<0.01);其中在CD4^+细胞中,TH1/TH2比值较健康对照者明显升高[(4.09±1.05)∶(3.12±0.89),P=0.003]。结论:AA患者外周血清中1,25-(OH)_2维生素D3水平异常降低,可能影响AA患者CD4^+T细胞的分化。Objective：To study the effect of 1,25-（OH）_2 D3 on T cell subsets in patients with aplastic anemia（AA）.Method：Fresh peripheral blood samples of 15 patients with AA and 25 normal controls were analyzed for the level of 1,25-（OH）_2 D3 with ELISA.The T cell subsets were detected by flow cytometry.Result：The median level of serum 1,25-（OH）_2 D3 of AA patients was lower than that of normal controls[（35.5±11.8）pmol/L vs.（51.8±12.6）pmol/L,P〈0.05].There was no significant difference of the median number of CD4~＋ cells between AA patients and normal controls[（31.8±4.7）% vs.（33.6±4.2）%,P=0.217].The median number of CD8~＋ cells in AA patients was higher than that in normal controls[（31.5±5.2）% vs.（24.8±3.8）%,P〈0.01].In CD4~＋ cells,the ratio of TH1/TH2 of AA patients was higher than that of normal controls [（4.09±1.05）vs.（3.12±0.89）,P=0.003].Conclusion：The level of serum 1,25-（OH）_2 D3 is decreased in AA patients,which may affect the differentiation of CD4~＋ T cell in AA patients.

关 键 词：1 25-(OH)2维生素D3 再生障碍性贫血 T细胞亚群 

分 类 号：R556.5[医药卫生—血液循环系统疾病]