超声波促进蛋白酶联合柠檬酸钠水解剩余污泥蛋白质的探究  

The effects of ultrasonic united sodium citrate with enzymatic treatment on protein release from the excess sludge

作　　者：邵艳珊 马欣[1] 王来福[1] 廉景燕[1] 

机构地区：[1]天津理工大学化学化工学院,天津300384

出　　处：《天津理工大学学报》2016年第2期61-64,共4页Journal of Tianjin University of Technology

摘　　要：本文主要研究超声波对蛋白酶藕合络合剂柠檬酸钠水解剩余污泥中的蛋白质含量的影响.通过凯氏定氮法来测提取液中蛋白质含量和场发射扫描电镜(FESEM)来观察来探究超声波、碱性蛋白酶等方法对剩余污泥中的团聚结构的破坏程度以及使蛋白质产生不同程度的解体.实验结果:超声处理后,协同络合剂柠檬酸钠(SC)与碱性蛋白酶共同对剩余污泥进行水解反应,当反应p H=8,T=50℃,碱性蛋白酶最佳量为60 mg/g,反应2.5 h时提取清液中的蛋白质浓度达到10 059 mg/L.This research aiming to investigate the soluble protein concentration from the excess sludge is by ultrasonic,adding chelator and protease. The different treatment mechanisms resulted in various degrees of biomass cell destruction,protein release,as evidenced by soluble protein concentration by Kjeldahl method and scanning electron microscopic imaging sludge.After ultrasonic treatment of residual sludge,carried out in cooperation with complexing agent SC with alkaline protease hydrolysis reaction,when p H = 8 reaction,reaction temperature T = 50 ℃,the concentration of alkaline protease for 60 mg/g,2.5 h reaction of protein content in the extract of 10 059 mg/L.

关 键 词：剩余污泥 超声波 碱性蛋白酶 络合剂 蛋白质 

分 类 号：X703[环境科学与工程—环境工程]