大连市学生HIV抗体确证试验结果分析  

Confirmatory test of HIV in students in Dalian during 2008-2014

作　　者：孙茂利[1] 刘大鹏[1] 熊邦杰 张群[1] 李德钧[1] 侯君[1] 王智勇[1] 

机构地区：[1]辽宁省大连市疾病预防控制中心,116021

出　　处：《中国学校卫生》2016年第3期414-417,共4页Chinese Journal of School Health

基　　金：大连市2015年医药卫生科学技术研究计划项目(2015079)

摘　　要：目的比较学生与非学生HIV抗体确证实验结果,为探讨学生艾滋病防控措施提供依据。方法对初筛送检的大连市HIV阳性样品采用免疫印迹法(WB)进行确认。对148份学生样品和2 675份非学生样品予以比较分析。结果学生首次检验阳性率(66.22%)低于非学生(77.12%);学生阴性率(0)低于非学生(9.27%);学生不确定率(33.78%)高于非学生(13.61%)。对不确定的进行追踪检验,学生失访率(0)明显低于非学生(46.98%);转阳率学生(100%)明显高于非学生(58.55%);学生转阴率(0)明显低于非学生(41.45%)。学生总阳性率(100%)高于非学生(81.35%),学生总阴性率(0)低于非学生(12.26%),学生总失访率(0)低于非学生(6.39%),差异均有统计学意义(χ2值分别为33.54,20.53,10.07,P值均<0.05)。学生人群中男性占98.65%,其中同性传播占90.41%;学生的不确定样品的带型,首次检验明显高的只有gp160(94.00%),p24(98.00%),其次是gp120(44.00%),检出率≤10%的蛋白共有5种。与首次检验直接阳性的相比,gp41,p31追踪检验转阳检出率较低,差异均有统计学意义(χ2值分别为14.40,22.49,P值均<0.01);其他7种蛋白的检出率相差不大。结论学生的HIV抗体确诊实验有诸多特点,首次检验不确定率高;追踪检验转阳率高;追踪检验转阳者,p31,gp41的最终检出率较低。Objective To compare the results of confirmatory test（ CT） of HIV between student and non- student（ NS）individuals and to provide basic information for HIV preventive strategies in students. Methods A total of 2 823 samples were underwent CT by Western Blotting（ WB）. Results In the first test（ FT）, positive rate of students（ 66. 22%） was lower than NS（ 77. 12%）, the negative rate of students（ 0） was lower than NS（ 9. 27%）, uncertainty rate（ UR） of students（ 33. 78%） was higher than NS（ 13. 61%）. In tracking test（ TT）, loss rate of students（ 0） was lower than NS（ 46. 98%）, transfer positive rate（ TPR） of students（ 100%） was higher than NS（ 58. 55%）, and transfer negative rate（ TNR） of students（ 0） was lower than NS（ 41. 45%）. In general, total positive rate（ TOPT） of students（ 100%） was higher than NS（ 81. 35%）, total negative rate（ TONT）of students（ 0） was lower than NS（ 12. 26%） and total loss rate of students（ 0） was lower than NS（ 13. 61%）. In FT, among 9kinds of the WB banding pattern（ BP） of uncertain samples among students, the detection rate（ DR） of gp160 was 94. 00%, p24 was 98. 00%, gp120 was 44. 00%, and there were 5 kinds that its DR was below 10%. Comparing with those samples that were judged positive directly in the FT, in the TT, the DR of gp41 and p31 were lower, other 7 kings of BP were not low. Conclusion There were wany characteristics in CT of student group, such as higher UR in FT; Higher TPR in TT, the DR of p31 and gp41 were lower among them who trunsfered to positive.

关 键 词：HIV抗体 获得性免疫缺陷综合征 学生保健服务 

分 类 号：R195[医药卫生—卫生统计学] R512.91[医药卫生—卫生事业管理]