一种产气纳米粒光声成像机制及体外成像的实验研究  

The Experimental Study of Gas-generating Nanoparticle in Photoacoustic Imaging Mechanism and Imaging in Vitro

作　　者：夏纪筑[1] 冯罡[1] 夏晓蓉[1] 郝兰[1] 王志刚[1] 

机构地区：[1]重庆医科大学超声影像学研究所超声分子影像重庆市重点实验室重庆医科大学附属第二医院超声科,重庆市400010

出　　处：《中国超声医学杂志》2016年第4期361-363,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金(No.81501481);重庆市博士后基金(No.Xm2014124)

摘　　要：目的通过体外实验观察脂质包裹的碳酸氢铵纳米粒用于光声成像的效果,并探讨其光声成像机制。方法应用红外光谱仪及光声成像仪分别检测各相关物质获得光谱图及光声信号图,阐明纳米粒成像机制;然后用光声成像仪检测纳米粒在不同时间、浓度、温度的变化情况。结果 (1)碳酸氢铵溶液近红外吸收峰约在1 300nm,包水纳米粒、氨水和葡萄糖约在1 100nm,双蒸水约在720nm,CO2约在700nm。(2)纳米粒随辐照时间,光声信号强度逐渐增大,然后再逐步降低;纳米粒浓度降低,产生光声信号强度及面积减小;纳米粒随温度升高,光声信号强度及面积增加。结论产气光声成像造影剂在体外具有光声成像的能力,并随时间、浓度、温度的增加可增强成像效果;CO_2可能是该造影剂光声成像的原因。Objective To observe the effect of nanoparticles of liposome encapsulating ammonium bicarbonate solution in photoacoustic imaging in vitro,and discuss the imaging mechanism.Methods First using infrared spectrometer and photoacoustic imaging system to test absorption spectra and photoacoustic signal images of various substances,and illuminate the imaging mechanism.Then using photoacoustic imaging system to test the change of ammonium in different time、concentration and temperature.Results（1）Absorption peak of ammonium bicarbonate solution is at about 1300 nm,absorption peaks of double distilled water-encapsulating nanoparticles,ammonia solution and5% glucose solution are at about 1100 nm,absorption peak of double distilled water is at about 720 nm,and CO2 absorption peak is at about 700 nm.（2）The photoacoustic signal intensity are gradually stronger first,then thin down gradually.The photoacoustic signal intensity and area weakened with concentration reduced.The photoacoustic signal intensity and area enhanced with temperature rise.Conclusions The gas-generating nanoparticles have ability of photoacoustic imaging in vitro,and which strengthen imaging effect with time、concentration and temperature.CO2 maybe the reason of imaging.

关 键 词：光声成像 产气 碳酸氢铵 纳米粒 造影剂 

分 类 号：R445.1[医药卫生—影像医学与核医学]