低强度超声联合氨基糖苷类药物对ESBLs大肠杆菌生物膜的影响  

Effects of Low-intensity Ultrasound Combined with Aminoglycosides on ESBLs E.coli Biofilm

作　　者：江和逊 苏杭[1] 郑慧敏[1] 董宇[1] 李岱容[1] 杜永洪[1] 

机构地区：[1]重庆医科大学生物医学工程学院省部共建国家重点实验室培育基地--重庆市超声医学工程重点实验室重庆市生物医学工程学重点实验室,重庆市400016

出　　处：《中国超声医学杂志》2016年第4期364-366,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家973计划项目(No.2011CB707900);国家自然科学基金项目(No.81101216;No.81127901;No.31571453)

摘　　要：目的探讨低强度超声联合氨基糖苷类药物对产超广谱β-内酰胺酶(ESBLs)大肠杆菌生物膜内细菌活性及生物膜渗透性的影响。方法超声单独以及分别联合庆大霉素或妥布霉素处理ESBLs大肠杆菌生物膜,测定实验前后生物膜内细菌活性、生物膜大体形态、生物膜对氨基糖苷类药物渗透作用的改变情况。结果 (1)经实验处理后,生物膜内细菌活性出现了明显的下降。(2)扫描电镜、共聚焦结果显示超声辐照前后生物膜结构改变明显。(3)低强度超声联合氨基糖苷类药物能明显增强生物膜对环丙沙星的渗透性。结论低强度超声联合氨基糖苷类药物对ESBLs大肠杆菌生物膜有显著的协同增效杀菌作用,且能改变其渗透性。Objective To study the effects of low-intensity ultrasound combined with aminoglycosides on bacterial vitality and penetration of extended-spectrum beta-lactamases（ESBLs）-producing Escherichia coli（E.coli）biofilms.Methods ESBLs E.coli biofilms were established in vitro,and irradiated by ultrasound with or without antibiotics.The morphology and vitality of bacteria were measured before and after the treatments.A ciprofloxacin penetration test was also conducted.Results（1）After treatment,the biofilms vitality experienced a significant decrease.（2）The results of scanning electron microscope and laser scanning confocal microscope showed that the biofilm morphology was changing.（3）The permeability of biofilmst to aminoglycosides was enhancing significantly after treatment.Conclusions Low-intensity ultrasound with aminoglycosides can enhance the permeability of biofilms,and produce different synergistic bactericidal effects.

关 键 词：生物膜活性 渗透性 低强度超声 氨基糖苷类药物 

分 类 号：R454.3[医药卫生—治疗学]