盐生杜氏藻烯醇酶的盐适应性及其与物质积累的关系  

Relationship between the Adaptability of Enolase to NaCl Stress and Substance Metabolism in Dunaliella salina

作　　者：郭金耀[1,2] 杨晓玲[1,2] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《淮海工学院学报（自然科学版）》2016年第1期70-73,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：江苏省优势学科建设工程资助项目(5511201401X-8)

摘　　要：为探索盐生杜氏藻烯醇酶的盐适应性及其与物质积累的关系，检测分析不同盐度下盐藻的生理生化变化．结果表明，在40～120g／L盐度范围内，烯醇酶活性与盐度呈正相关；在120～200g／L盐度范围内，烯醇酶活性与盐度呈负相关；盐度为120g／L时，烯醇酶活性最大；烯醇酶活性与盐藻叶绿素形成、蛋白质积累和细胞生长均呈正相关性．盐藻烯醇酶是一种高盐适应酶，在其活性最高时，盐藻生长最快．The paper has analyzed relationship between adaptability of enolase to NaCl stress and the substance metabolism in Dunaliella salina under different NaCl stress. The result showed that the enolase activity was positive correlation with NaCl concentration in the enhancement of NaCl concentration from 40 to 120 g/L, but the enolase activity was negative correlation with NaCl concentration with the enhancement of NaCl concentration from 120 to 200 g/L. When NaCl concentration was 120 g/L, enolase activity was the highest. It has been proved that the enolase is a kind of enzymes which can adapt well to NaCl stress in D. salina. The relationship of the enolase activity and cell density, chlorophyll formation, accumulation of protein is positive. When enolase activity was highest, substance metabolism was also highest in D. salina.

关 键 词：盐藻 烯醇酶 盐度 生长 

分 类 号：Q945.78[生物学—植物学]