microRNA-155在寻常型银屑病中的表达及意义  

Expression andsignificance of miR-155 in psoriasis vulgaris

作　　者：贺琪[1] 皮先明[1] 石全[1] 李家文[2] 杨迎香 陈宏翔[2] 

机构地区：[1]湖北省中医院皮肤科,武汉市430061 [2]华中科技大学同济医学院附属协和医院皮肤科,武汉市430022 [3]湖北省浠水县中医院皮肤科,438200

出　　处：《实用医学杂志》2016年第6期900-903,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81171495;81271765)

摘　　要：目的:探讨micro RNA-155(mi R-155)在寻常型银屑病中的表达及意义。方法:采用Taq Man探针实时荧光定量聚合酶链式反应(q RT-PCR)检测35例寻常型银屑病患者皮损区(皮损组)和非皮损区(非皮损组)中的mi R-155表达水平,并与30例正常对照(对照组)进行比较,研究mi R-155含量与患者银屑病面积和严重程度指数(PASI)评分和白细胞介素-17A(IL-17A)含量之间的关系。结果:mi R-155和IL-17A在皮损组和非皮损组中的表达高于对照组,在皮损组中的表达高于非皮损组,差异均有统计学意义(均P<0.01);皮损组中,mi R-155和IL-17A含量分别与PASI评分呈显著正相关(均P<0.05),mi R-155和IL-17A含量之间亦存在显著正相关(P<0.01)。结论:mi R-155的过表达与银屑病的发病有关,银屑病中mi R-155的过表达与Th17细胞的功能亢进有关。Objective Toexplore the expression and significance of micro RNA-155（mi R-155） in psoriasis vulgaris. Methods Areal-time quantitative reverse transcription polymerase chain reaction（q RT-PCR）method with Taq Man probe technology was performed to detect mi R-155 expression in skin lesion area and nonskinlesionalarea of 35 patients with psoriasis vulgaris, compared with that of 30 normal controls. The correlations among mi R-155 expression, psoriasis area and severity index（PASI） score and Interleukin-17A（IL-17A）expression were studied. Results The expressions of mi R-155 and IL-17 A in lesional and non-lesionalgroups were higher than that of control group（all P〈0.01）. Also expressions in lesional skin were higher than nonlesional skin（both P〈0.01）. In skin lesion group, significant positive correlations existed betweenmi R-155 or IL-17 A expression and PASI score as well as mi R-155 and IL-17 A expression（all P〈0.05）. Conclusions Upexpression of mi R-155 was relevant to psoriasis development, which is related withthe hyperfunctionof Th17 cells in psoriasis.

关 键 词：MIR-155 寻常型银屑病 TAQMAN探针 白细胞介素-17 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]