基于DNA条形码和~1H-NMR代谢组学二维方法的多基原藏药沙棘鉴定  

Genetic and chemical discrimination of traditional Tibetan medicine seabuckthorn based on DNA barcode and ~1H-NMR metabolic method

作　　者：刘悦[1] 刘川[1] 谭尔[1] 范刚[1] 向丽[2] 李晓东[3] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]中国中医科学院中药研究所,北京100700 [3]中国食品药品检定研究院,北京100050

出　　处：《中国中药杂志》2016年第4期578-585,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473428);四川省教育厅省属高校科研创新团队建设计划项目(11TD004);四川省科技支撑计划项目(2013SZ0114)

摘　　要：沙棘为典型多基原藏药,混用现象时有发生。该文旨在构建一种基于DNA条形码和~1H-NMR代谢组学的二维鉴定方法,为沙棘药材的准确鉴定与应用提供参考。应用ITS2和psb A-trn H序列以对中国沙棘、江孜沙棘和西藏沙棘进行DNA条形码鉴定,分析其种内及种间变异、遗传距离等,构建系统发育邻接(NJ)树。同时采用基于~1H-NMR代谢组学方法获得3种沙棘药材的代谢物指纹谱图,对所得数据进行主成分分析(PCA)。结果表明,DNA条形码结合~1H-NMR代谢组学可以发掘3种沙棘种间的遗传和化学特征的差异性,并对3种沙棘进行准确鉴定。基于DNA条形码和~1H-NMR代谢组学建立的多基原藏药沙棘二维鉴定方法准确可靠,能为藏药鉴定和质量评价提供了一种新思路。To differentiate three medicinal Hippopahe species of seabuckthorn,a combined genetic and chemical identification method was established in this study. ITS2 and psb A-trn H were tested for identification of 3 species of seabuckthorn. Detection of the kimura2-parameter（ K2P） distance,the neighbor-joining（ NJ） tree and the barcoding gap were used to assess the identification efficiency.~1H-NMR based metabolic method was applied to acquire the profile of metabolites. PCA was used to analysis the metabolite data. The results indicated that DNA barcode combined^1H-NMR based metabolic method is a powerful tool for the identification of 3 medicinal Hippopahe species of seabuckthorn. The finding demonstrated that different genetic variation and chemical constituents existed among 3medicinal Hippopahe species of seabuckthorn. The combined identification method will improve the reliability of species discrimination and could be applicable to much other ethnic medicine which has various origins in China.

关 键 词：藏药 沙棘 DNA条形码 1H-NMR代谢组学 二维鉴定方法 

分 类 号：R29[医药卫生—民族医学]