藏药小檗皮的质量标准研究  

Study on quality standard of Berberidis Cortex

作　　者：李艳[1] 吕秀梅[1] 林亚丽[1] 莫家祺 范刚[1] 赖先荣[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137

出　　处：《中国中药杂志》2016年第4期592-596,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81303310;81173360);高等学校博士点专项科研基金(新教师类)联合项目(20135132120012)

摘　　要：为建立藏药小檗皮的质量标准,提高其质量控制水平,该研究参照《中国药典》2010年版附录相关方法,测定小檗皮药材的水分、灰分和浸出物的含量;以主要有效成分木兰花碱、药根碱、巴马汀、小檗碱为指标,分别采用薄层色谱法（TLC）和高效液相色谱法（HPLC）进行定性鉴别和含量测定研究。结果该药材的TLC鉴别斑点清晰、分离度较好;18批药材的测定结果表明水分为8.39%~12.23%,总灰分为4.50%~9.96%,酸不溶性灰分为0.10%~0.69%,浸出物为20.62%~37.13%,药材中木兰花碱、药根碱、巴马汀、小檗碱的平均质量分数分别为5.98%,0.63%,0.30%,2.50%。该研究所建立的方法准确可靠,可用于小檗皮药材的质量控制和质量评价。In order to establish the quality standard of Berberidis Cortex and improve its quality control level,water,total ash,acid-insoluble ash and alcohol-soluble extract were determined according to procedures recorded in the Chinese Pharmacopoeia（ 2010edition）. The qualitative and quantitative analyses were performed by thin layer chromatography（ TLC） and high performance liquid chromatography（ HPLC） methods. The results showed that TLC identification had a good resolution with clear spots. The water content was 8. 39%-12. 23%; total ash was 4. 50%-9. 96%; acid-insoluble ash was 0. 10%-0. 69%,and the alcohol-soluble extraction was20. 62%-37. 13%. The average contents of magnoflorine,jatrorrhizine,palmatine,and berberine in Berberidis Cortex were 5. 98%,0. 63%,0. 30%,2. 50%,respectively. It was concluded that the developed method was accurate and good in specificity,which can be used for quality control of Berberidis Cortex in the future.

关 键 词：小檗皮 质量标准 木兰花碱 药根碱 巴马汀 小檗碱 

分 类 号：R29[医药卫生—民族医学]