藏药蔓菁膏抗缺氧作用的量-效/毒关系及质量标准研究  

Studies on dosage-efficacy/toxicity relationship of anti-hypoxia effects and quality standard of Tibetan medicine Brassicea Radix extract

作　　者：邝婷婷[1] 王宇[1] 王张[1] 张艺[1] 赖先荣[1] 范刚[1] 王毓杰[1] 游佳莉[1] 陈一龙[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137

出　　处：《中国中药杂志》2016年第4期597-602,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81403187);四川省食品药品监督管理局科研课题(301-377);四川省中医药管理局科技专项(2012-G-029);成都中医药大学科技发展基金项目(CGZH201201)

摘　　要：为评价藏药蔓菁膏抗缺氧作用的有效性和安全性,并建立蔓菁膏质量标准。该文采用急性毒性和亚急性毒性初步评价安全性;采用小鼠常压耐缺氧、小鼠急性脑缺血性缺氧、小鼠亚硝酸钠中毒模型研究蔓菁膏的抗缺氧作用;参照《中国药典》2010年附录相关方法对不同批次蔓菁膏的水分、灰分、水中不溶物进行测定,采用蔓菁对照药材进行了薄层色谱鉴别,采用苯酚-硫酸法测定蔓菁膏中多糖的含量。结果表明:1小鼠经口给予蔓菁膏的LD50为56.73 g·kg-1,Dm为86.80 g·kg-1·d-1,Dn为35.55 g·kg-1·d-1;2蔓菁膏具有提高缺氧耐受力的作用,确定有效剂量为0.388 g·kg-1·d-1;3建立了蔓菁膏薄层鉴别和多糖含量测定方法。所建立的质量控制方法操作、准确、简便,制定标准限度合理,已被《四川省藏药材标准》收载,同时蔓菁膏具有明显的抗缺氧作用且安全性良好,为蔓菁膏的临床安全有效用药和保健品开发提供了重要依据。To evaluate the efficacy and safety associated with anti-hypoxia effect and establish the quality standard for Brassicea Radix extract,the investigations of acute toxicity and subacute toxicity were carried out to preliminarily appraise the toxicity,and the models of normal pressure hypoxia,acute cerebral ischemia and sodium nitrite poisoning in mice were used to evaluate the effect of enhancing anoxia endurance. Then according to the methods described in the Appendix of Chinese Pharmacopoeia（ 2010 edition）,the sulfuric acid-phenol method was applied to determine the content of polysaccharide,and the water,ash and insoluble matter in water inspections were carried out and the control medicinal herb was identified with the samples by qualitative TLC. The results indicated that 1the toxic effects（ LD50） of mice was 56. 73 g·kg- 1by oral administration of Brassicea Radix extract,while Dm and Dn were respective 86. 80 g·kg·d- 1and 35. 55 g·kg·d- 1; 2the determined effective dosage of Brassicea Radix extract which could enhance anoxia endurance was 0. 388 g·kg- 1·d- 1; 3 the methods of TLC and the content of polysaccharide were established. The method of quality control has been recorded in Sichuan Province Standard for Tibetan Medicine,which is reliable,accurate and simple,with good reproducibility. Meanwhile,given the prominent effect on anti-hypoxia and good safety,it provided important basis for clinic safe and effective usage and the development of health products.

关 键 词：蔓菁膏 抗缺氧 量-效/毒关系 质量标准 藏药 

分 类 号：R29[医药卫生—民族医学]