104份甘肃小麦品种主要品质基因的分子标记检测  

Molecular Marker Characterization of Main Quality Genes in 104 Gansu Wheat Varieties

作　　者：柳娜[1] 曹东[2] 王世红[1] 杨文雄[1] 

机构地区：[1]甘肃省农业科学院小麦研究所,兰州730070 [2]甘肃农业大学农学院,兰州730070

出　　处：《西北农业学报》2016年第3期353-360,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31560390);公益性行业(农业)科研专项(201503125-1);甘肃省小麦工程技术研究中心专项(144JTGA230);国家农业科技成果转化资金(2014GB2G100140)~~

摘　　要：高分子量麦谷蛋白亚基、黄色素质量分数和1B/1R易位系均影响小麦的加工品质。为明确相关品质性状基因在104份甘肃育成小麦品种中的分布,并为小麦品质育种提供亲本材料,利用高分子质量谷蛋白亚基Dx5、Bx7、By8、By9和黄色素质量分数基因及1B/1R易位系的特异性标记对104份小麦品种进行检测。结果表明,含基因Dx5、Bx7、By8、By9和1B/1R易位系的品种分别占总品种数的63.46%、76.92%、52.88%、45.19%和45.19%。含有等位基因Psy-A1a和Psy-A1b品种分别占总品种数的60.58%和21.15%,其黄色素质量分数均值差异达到显著水平(P<0.05)。总体来看,甘肃育成小麦品种中含适合加工馒头和面粉等传统食品的单个品质优质基因频率较高,而优质基因组合的比例较低,具有黄色素质量分数较高的品种比例高。Subunits of high molecular weight glutenin,yellow pigment mass fraction and 1B/1Rtranslocation have main impacts on wheat processing quality.In this study,locus specific molecular markers for high molecular mass glutenin subunits of Dx5,Bx7,By8 and By9,PSY,1B/1Rtranslocation genes were used to characterize 104 wheat varieties to know the distribution of these genes in wheat varieties of Gansu province,and provide parent materials in molecular marker assisted breeding program for wheat with good quality.The results showed that,the frequency of cultivars with genes of Dx5,Bx7,By8,By9 and 1B/1R translocation were 63.46%,76.92%,52.88%,84.62%,45.19%and 45.19%,respectively.The proportion of the allelic variations of Psy-A1 aand Psy-A1 bin these varieties were 60.58% and 21.15%respectively.Significant differences in yellow pigment mass fraction were detected between the genotypes with Psy-A1 aand those with Psy-A1b（P〈0.05）.Generally,the frequency of single good quality gene for processing traditional food,such as steamed bread and flour,was high,the combination of good quality genes were low and the number of cultivars with high yellow pigment mass fraction was high in wheat cultivars released in Gansu province.

关 键 词：甘肃小麦 高分子量谷蛋白亚基 品质基因 分子标记 

分 类 号：S512.1[农业科学—作物学]