青藏高原蕨麻种质资源遗传多样性POD同工酶分析  

Genetic Diversity of Potentilla anserinain Qinghai-Tibetan Plateau of China Based on Peroxidase Isozyme

作　　者：刘贺贺[1,2] 蒋红霞[1,2] 富贵[1,2] 白世俊[1,2] 包锦渊[1,2] 韦梅琴[3] 李军乔[1,2] 

机构地区：[1]青海民族大学化学化工学院,西宁810007 [2]青海省生物技术与分析测试重点实验室,西宁810007 [3]青海大学农牧学院,西宁810016

出　　处：《西北农业学报》2016年第3期413-422,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：科技部农业科技成果转化基金(2010GB2G00514);国家自然科学基金(30607026;30660019);青海省自然科学基金(2012-Z-907)~~

摘　　要：采用聚丙烯酰胺凝胶电泳技术分析75份蕨麻过氧化物同工酶,结果与形态学标记、细胞水平、分子标记研究的结果一致,显示蕨麻的遗传多样性丰富。证实蕨麻具有丰富遗传变异,为合理保护与科学利用蕨麻资源提供科学依据。检测显示,蕨麻叶片过氧化物同工酶电泳共出现14条酶带;酶带多态位点百分率为100%;NTSYSpc 2.1软件计算的遗传相似系数为0.214～1.000,平均值为0.681;以遗传相似系数为基础,采用非加权类平均法进行聚类分析,在相似系数为0.67时分为2大类,第1类为蕨麻,第2类为鹅绒委陵菜。蕨麻种质资源遗传多样性及遗传变异均较丰富。说明,蕨麻丰富的遗传多样性是在青藏高原复杂的地理环境中长期进化的结果。We used technique of polyacrylamide gel electrophoresto analyze the peroxidase isozyme in75 Potentilla anserina.The results showed that biochemical markers were consistent with the results in morphological markers,cell level and molecular markers,and Potentillaanserinehad genetic diversity.By peroxidase isoenzyme electrophoresis,we found 14 enzyme bands of Potentilla anserina leaf;percentage of polymorphic bands was 100%;genetic similarity coefficient calculated by the software NTSYSpc 2.1ranged from 0.214 to 1.000,and the average value was 0.681;Based on the genetic similarity coefficient,cluster analysis was done by method of unweighted pair group method with arithmetic mearns used Potentilla anserina material,Potentilla anserina was divided into two categories when the genetic similarity was 0.67,the main one of the first category was Potentilla anserina material,the main one in the second category was Potentilla anserine material.In conclusion,rich genetic diversity and variations of Potentilla anserina was caused by evolution in the complicated geographical environment of the Qinghai Tibet Plateau in long time.

关 键 词：蕨麻 遗传多样性 过氧化物同工酶 聚丙烯酰胺凝胶电泳 相似系数 多态位点百分率 

分 类 号：S567.239[农业科学—中草药栽培]