磷酸化c—Jun氨基末端激酶和P38丝裂原活化蛋白激酶在寻常性银屑病皮损中的表达  

Expressions of phosphorylated c-Jun N-terminal kinase and P38 mitogen-activated protein kinase in psoriasisvulgaris lesions

作　　者：葛新红[1] 唐真真 焦亚宁[1] 吴昊[3] 喻楠[1] 董灵娣[1] 李乐[1] 杨彪[3] 蒲小霞[3] 

机构地区：[1]宁夏医科大学总医院皮肤科,银川750004 [2]山东省兰陵县人民医院皮肤科 [3]宁夏医科大学

出　　处：《中华皮肤科杂志》2016年第4期248-251,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81060181）;宁夏自然科学基金（NZ14121）

摘　　要：目的探讨磷酸化c—Jun氨基末端激酶（p—JNK）和P38丝裂原活化蛋白激酶（p—P38MAPK）在寻常性银屑病皮损中的表达。方法收集30例确诊的寻常性银屑病患者皮损，同时收集30例健康人皮肤组织作为对照组。采用免疫组化及Western印迹法分别检测p-JNK和p-P38MAPK蛋白在寻常性银屑病皮损组织和正常人皮肤中的表达情况。结果免疫组化结果显示，寻常性银屑病皮损组织p-JNK和p-P38MAPK表达的平均吸光度（AOD）值分别为0．663±0．016和0．436±0．011，均明显高于对照组（0．333±0．009和0．306±0．010），差异有统计学意义（t=44．869、21．913，均P〈0．001）。Western印迹法同样显示，寻常性银屑病皮损p-JNK和p-P38MAPK蛋白相对表达量均显著高于对照组，差异均有统计学意义（t值分别为20．477和165．084，均P〈0．05）。结论JNK和P38MAPK的活化可能参与了寻常性银屑病表皮细胞的过度增殖。Objective To investigate expressions of phosphorylated c-Jun N-terminal kinase （p-JNK） and P38 mitogen-activated protein kinase （p-P38MAPK） in psoriasis vulgaris lesions. Methods Tissue specimens were obtained from lesions of 30 patients with psoriasis vulgaris and normal skin of 30 healthy human controls. An immunohistochemical study and Western-blot analysis were performed to measure protein expressions of p-JNK and p-P38MAPK in these skin specimens. Results As the immunohistochemical study showed, the expressions of p-JNK and p-P38MAPK （expressed as the average optical density [ AOD ] value for targeted proteins） were significantly higher in psoriasis vulgaris lesions than in normal skin tissues （p-JNK： 0.663 ± 0.016 vs. 0.333 ± 0.009, t = 44.869, P〈 0.001; p-P38MAPK： 0.436 ± 0.011 vs. 0.306 ±0.010, t = 21.913, P 〈 0.001 ）. Western-blot analysis also showed increased protein expressions of p-JNK and p-P38MAPK in psoriasis vulgaris lesions compared with normal skin tissues （t = 20.477, 165.084, respectively, both P 〈 0.05 ）. Conclusion The activation of JNK and P38MAPK may be involved in the overproliferation of epidermal cells in psoriasis vulgaris lesions.

关 键 词：银屑病 JNK丝裂原活化蛋白激酶类 P38丝裂原活化蛋白激酶类 c—Jun氨基末端激酶 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]