HRP-F1McAb酶免疫染色技术检测小鼠脏器鼠疫F1抗原应用效果评价  

The evaluation of the application effect of the HRP-F1 McAb enzyme immunostaining technique for detection of F1 antigen of Yersinia pestis in the mice organs

作　　者：白雪薇[1] 刘合智[1] 周松[1] 王海峰[1] 牛艳芬[1] 陈凯乐 史献明[1] 杜国义[1] 

机构地区：[1]河北省鼠疫防治所,河北张家口075000

出　　处：《国外医学（医学地理分册）》2016年第1期70-72,共3页Foreign Medical Sciences：Section of Medgeography

基　　金：河北省卫生厅青年科技资助项目(No:20120033)

摘　　要：目的评价辣根过氧化酶标记鼠疫F1单克隆抗体(HRP-F1McAb)酶免疫染色技术检测小鼠脏器鼠疫F1抗原的应用效果。方法通过HRP-F1McAb酶免疫染色法检测鼠疫菌EV株感染小鼠脏器标本和对照小鼠脏器标本,同时用细菌学、RIHA和ELISA做平行检测。结果 HRP-F1McAb酶免疫染色法与细菌学一致率为96.36%,阳性检出率差异无统计学意义(χ^(2=0),P>0.05);HRP-F1McAb酶免疫染色法和RIHA一致率为98.18%,阳性检出率差异无统计学意义(χ^(2=0),P>0.05);HRP-F1McAb酶免疫染色法和ELISA一致率为96.36%,阳性检出率差异无统计学意义(χ^(2=0),P>0.05)。结论酶免疫染色法检测小鼠脏器鼠疫F1抗原特异敏感、简单快速。Objective To evaluate the application effect of the experiment of horseradish peroxidase labeled plague F1 monoclonal antibody（HRP-F1McAb）enzyme immunostaining Technique for detection of F1 antigen of Yersinia pestis in the mice organs.Methods Viscera organ specimens of mice infected with Y.pestis of EV and control specimens of mice were detected by HRP-F1 McAb enzyme immunostaining technique,meanwhile,bacteriological method RIHA and ELISA were carried out for parallel testing.Results Coincidence was 96.36%between HRP-F1 McAb enzyme immunostaining technique and bacteriological method,and the difference was statistically insignificant in the positive detection rates（χ^2=0）,P0.05）;Coincidence was 98.18% between HRPF1 McAb enzyme immunostaining technique and RIHA,with statistically insignificant difference in the positive detection rates（χ^2=0）,P 0.05）.Coincidence was 96.36% between HRP-F1 McAb enzyme immunostaining technique and ELISA,and the difference was statistically insignificant in the positive detection rates（χ^22=0）,P0.05）;Conclusion The enzyme immunostaining technique is specific and sensitive,simple and fast in detection of F1 antigen of the plague.

关 键 词：HRP-F1McAb酶免疫染色技术 鼠疫耶尔森菌 F1抗原 

分 类 号：R591[医药卫生—内科学]