IL-18及IL-18结合蛋白、IFN-γ等血清炎性介质在囊型包虫病致Th1/Th2细胞偏移中的意义  

The potential significance of IFN-γ,IL-18 and IL-18BP in Th1/Th2 3 caused in patients with hepatic cystic echinococcosis

作　　者：郭忠帅 张峰波[2] 向阳[2] 林雨薇[3] 庞楠楠[2] 赵慧[2] 张佳[1] 马秀敏[2] 丁剑冰[1] 

机构地区：[1]新疆医科大学基础医学院免疫教研室,乌鲁木齐830011 [2]新疆医科大学第一附属医院医学检验中心,乌鲁木齐830054 [3]新疆医科大学附属中医医院临床检验中心,乌鲁木齐830000

出　　处：《新疆医科大学学报》2016年第4期430-432,436,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81460307;81160200;81060135;81160378);新疆自治区包虫病重点实验室开放课题(XJDX0202-2010-04)

摘　　要：目的探讨联合检测血清中白介素18(IL-18)和白介素18结合蛋白(IL-18BP)在囊型包虫病致Th1/Th2细胞偏移发生和发展中的作用。方法以25例健康者为HC组,28例囊性包虫病患者为CE组,28例囊性包虫病术后患者为PCE组。采用酶联免疫吸附法检测3组患者血清IL-18、干扰素γ(IFN-γ)、白介素2(IL-2)和IL-18BP、白介素4(IL-4)水平;采用免疫比浊法检测3组患者血清C反应蛋白(CRP)、补体3(C3)、补体4(C4)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、免疫球蛋白E(IgE)水平。结果 CE组血清IL-18、IFN-γ、IL-2、C3及C4水平明显低于HC组和PCE组,差异有统计学意义(P<0.05);CE组血清IL-18BP、IL-4、CRP、IgM、IgG及IgE水平明显高于HC组和PCE组,差异有统计学意义(P<0.05)。结论血清IFN-γ、IL-18及IL-18BP在肝囊型包虫病所致的Th1/Th2偏移发生和发展过程中具有协同及相互制约的作用。Objective To investigate the significance of combined detection of the serum levels of interleukin 18（IL-18）and interleukin 18 binding protein（IL-18BP）in hepatic cystic echinococcosis caused by Th1/Th2 imbalance.Methods 28 patients with cystic echinococcosis as CE group,28 patients with cystic echinococcosis preoperative surgery as PCE group,25 cases of healthy persons as HC group,Enzyme-linked immunosorbent method to detect the serum IL-18 and IL-18 BP and Th cytokines in each group;Immune turbidimetric method to detect the serum CRP,C3,C4 and IgG,IgM,IgE level in each group.Results（1）Serum IL-18,IFN-γ,IL-2,C3 and C4concentrations were remarkably lower in CE compared with HC and PCE group（P〈0.05）.（2）Serum IL-18 BP,IL-4,CRP,IgE,IgM and IgG concentration was higher CE compared with HC and PCE group（P〈0.05）.Conclusion The imbalance of IL-18 and IL-18 BP may one of the possible mechanisim that echinococcus granulosusused to actively modulate and suppress host immune system so that Th1/Th2 immune offset.

关 键 词：肝囊性包虫病 白细胞介素18 白细胞介素18结合蛋白 

分 类 号：R392.1[医药卫生—免疫学]