2型糖尿病性视网膜病变PBMCs中miR-126表达的研究  

Expression of miR-126 in PBMCs of diabetic retinopathy patients

作　　者：高祎[1] 刘玉震[1] 王丽君[1] 房炳华[1] 雷宁玉[1] 

机构地区：[1]滨州医学院附属医院眼科,山东256603

出　　处：《中国实用眼科杂志》2016年第4期322-325,共4页Chinese Journal of Practical Ophthalmology

摘　　要：目的探讨糖尿病性视网膜病变（DR）患者外周血单个核细胞（PBMCs）中miR-126的水平变化及其临床意义。方法临床病例对照研究。对2014年12月到2015年3月在滨州医学院附属医院内分泌科及眼科住院的76例患者分为无糖尿病性视网膜病变组（NDR组）30例，非增殖期糖尿病性视网膜病变组（NPDR组）25例，增殖期糖尿病性视网膜病变组（PDR组）21例，及正常对照组（CON组）30名。收集患者的-般检查结果，用逆转录PCR（RT—PCR）检测PBMCs中miR-126的水平，及酶联免疫吸附法（ELISA法）检测血浆中VEGF的水平。结果高密度脂蛋白（HDL）在PDR组明显高于NDR组和NPDR组，差异有统计学意义（P〈0．05）。PDR组的白细胞（WBC）数明显比其余三组高，差异有统计学意义（P〈0．05）。miR-126在NPDR组、PDR组明显低于CON组和NDR组，差异有统计学意义（P〈0．05）。与CON组相比，血浆中的VEGF在NDR组、NPDR组和PDR组中明显上调，差异有统计学意义（P〈0．05）。PBMCs中miR-126水平与FPG、VEGF差异有统计学意义（P〈0．05），（r=-0．67，P〈0．05，f=-0．74，P〈O．05）。结论PBMCs中miR-126参与到DR的发生及发展的过程，其水平变化与病程有关。Objective To investigate the expression of miR-126 and the clinical significance in peripheral blood mononuclear cells （PBMCs） of patients with diabetic retinopathy （DR）. Methods Seventy-six type 2 diabetes mellitus （DM） patients were assigned into four groups： non-diabetic reti- nopathy （NDR, n=30）, non-proliferative diabetic retinopathy （NPDR, n=25）, proliferative diabetic reti- nopathy （PDR, n=21） and control （CON, n=30）. By RT-PCR, the expression of miR-126 in PBMCs was detected and the expression of plasma VEGF was analyzed using ELISA. Results The plasma level of high density lipoprotein （HDL） and the number of white blood cell （WBC） in PDR group was significant higher than that in NDR and NPDR groups （P 〈0.05）. The expression of miR-126 in PDR and NPDR groups was lower than that in the NDR and CON groups （P 〈0.05）. Compared with the CON group, ELISA showed a significant increasing of plasma VEGF in the NDR, NPDR and PDR groups （P 〈0.05）. Moreover, there was a negative correlation between miR-126 in PBMCs and fasting plasma glucose （FPG） or VEGF （r =-0.67, P 〈0.05; r =-0.74, P 〈0.05）. Conclusions The changes of miR-126 in PBMCs may participate in the onset and development of DR, and asso- ciated with the seriousness of DR.

关 键 词：2型糖尿病 糖尿病性视网膜病变 PBMCS MIR-126 

分 类 号：R587.2[医药卫生—内分泌]