石菖蒲不同极性部位对抑郁模型小鼠抗抑郁效应及其机制  

Antidepressant Effect and Mechanism of Different Polar Fraction of Acorus Tatarinowii Schott. on Depression Mouse Model

作　　者：周天[1] 李辉[1] 凃中一 周威[1] 

机构地区：[1]武汉轻工大学医学技术与护理学院,武汉430023

出　　处：《医药导报》2016年第4期327-330,共4页Herald of Medicine

基　　金：湖北省教育厅科学技术研究计划重点项目(D20131702);湖北省卫生厅科研项目(JX6B100)

摘　　要：目的研究石菖蒲不同极性部位对模型小鼠抗抑郁效应,并探讨其可能的机制。方法采用系统溶剂法制备石菖蒲不同极性部位。选取《中华人民共和国药典》(简称《药典》)成人用推荐剂量10 g·d-1折算成对应的小鼠用量进行灌胃给药。将80只雄性SD小鼠随机分为正常对照组、模型对照组、石菖蒲不同极性部位组,采用慢性轻度不可预见刺激(CUMS)结合孤养建立小鼠抑郁模型,观察造模后小鼠的外在表现。饲养21 d后眼眶采血,采用ELISA法测定血中三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)、促甲状腺激素(TSH)、促肾上腺皮质激素(ACTH)的含量;5-羟色胺及ACTH在脑组织中的含量。结果模型对照组小鼠血浆中T3、T4、ACTH[(1.203±0.042),(44.80±2.21),(11.27±0.50)ng·m L-1]含量较正常对照组小鼠[(0.794±0.028),(24.87±1.25),(7.04±0.24)ng·m L-1]显著升高(P<0.05);脑组织中5-HT含量(146.87±10.96)ng·L-1较正常对照组(237.11±21.87)ng·L-1显著降低,ACTH浓度(58.94±4.46)ng·L-1较正常对照组(38.89±2.26)ng·L-1显著升高(P<0.05)。在《药典》推荐剂量下,石菖蒲乙醇组、环己烷组、三氯甲烷组血浆中T3[(0.824±0.067),(0.812±0.051),(0.943±0.049)ng·m L-1]、T4[(25.97±1.96),(27.53±1.88),(31.26±1.97)ng·m L-1]、ACTH[(7.21±0.40),(7.58±0.39),(8.69±0.42)ng·m L-1]较模型对照组含量明显降低;脑组织中5-HT[(219.59±10.48),(202.19±11.95),(186.96±10.29)ng·L-1]较模型对照组含量显著升高,ACTH含量[(41.65±2.65),(44.87±2.95),(47.75±3.06)ng·L-1]较模型对照组含量显著降低(P<0.05)。结论 CUMS抑郁模型小鼠表现出典型的抑郁症状,且与T3、T4、ACTH、5-HT等内分泌激素具有关联性。石菖蒲提取物及其低极性部位(环己烷、三氯甲烷组)在《药典》推荐剂量下能部分纠正抑郁小鼠内分泌失常,是石菖蒲具备抗抑郁效应的因素之一。Objective To study antidepressant effect and mechanism of different polar fraction of Acorus tatarinowii Schott.on depression mouse model and explore the mechanism. Methods Different polar fractions were prepared by systematic solvent method. The adult dose recommended by Chinese Pharmacopeia（ 10 g · d^-1） was converted to the dose of mouse.Intragastric administration was performed.A total of 80 male mice was randomly divided into normal control group,model control group and different polar fraction groups of Acorus tatarinowii Schott.. Depression mouse model was established by chronic unpredictable mild stimulation（ CUMS） with solitary feeding,and external performance of mice of different groups was observed.After 21-day feeding,blood was harvested from eyes of the mice.Concentrations of triiodothyronine（ T3）,tetraiodothyronine（ T4）,thyroid-stimulating hormone（ TSH）,adrenocorticotrophic hormone（ ACTH） in mouse plasma and 5-hydroxy tryptamine（ 5-HT）,ACTH in brain were measured by ELISA method. Results Concentrations of T3,T4 and ACTH in model control group[（ 1.203±0.042）,（ 44.80±2.21）,（ 11.27±0.50） ng·mL^-1] were significantly increased as compared with the normal control group [（ 0.794±0.028）,（ 24.87±1.25）,（ 7.04±0.24） ng·mL^-1]（ P〈0.05）.Concentration of 5-HT in brain（ 146.87±10.96）was significantly decreased as compared with that of normal group（ 237. 11 ± 21. 87） ng · L-1,and concentration of ACTH（ 58.94±4.46） ng·L^-1was significantly increased as compared with that of normal group [（ 38.89±2.26） ng·L^-1,P〈0.05） ].Concentrations of T3[（ 0.824±0.067）,（ 0.812± 0.051）,（ 0.943± 0.049） ng·mL^-1],T4[（ 25.97± 1.96）,（ 27.53± 1.88）,（ 31.26±1.97） ng·mL^-1]and ACTH [（ 7.21±0.40）,（ 7.58±0.39）,（ 8.69±0.42） ng·mL^-1]in extraction group,cyclohexane group and chloroform group were significantly decreased as compared with model control group. At the recommendation dose of Chinese Pharmac

关 键 词：石菖蒲 不同极性部位 抗抑郁 内分泌激素 

分 类 号：R285.5[医药卫生—中药学] R749[医药卫生—中医学]