正交试验法优化珠子参总皂苷提取工艺  

Optimization of extraction technology for total saponins from Rhizoma Panacis Majoris using orthogonal test

作　　者：李凤英[1] 王英姿[1] 王晴[1] 张秀婷[1] 张春泥[1] 李文华[1] 骆声秀 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2016年第2期151-154,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家科技重大专项课题(No.2012ZX09103201-044)

摘　　要：目的优选珠子参总皂苷的提取工艺,为珠子参中皂苷类化合物的开发利用提供理论依据。方法采用紫外分光光度法建立珠子参中总皂苷含量的测定方法,检测波长540 nm,并通过正交试验考察乙醇浓度、溶剂倍量、提取时间及提取次数对珠子参总皂苷提取工艺的影响。结果珠子参总皂苷的线性范围为0.052~0.182 mg,平均加样回收率为98.64%。珠子参总皂苷最佳提取工艺为加入70%的乙醇10倍量,提取3次,每次提取1 h,平均收率12.32%。结论建立的紫外分光光度法检测珠子参总皂苷含量方法准确可靠,优选的珠子参总皂苷提取工艺简便、稳定可行。Objective To optimize extraction technology for total saponins from Rhizoma Panacis Majoris（ zhuzishen） and provide a theoretical basis for development and utilization of saponins from Rhizoma Panacis Majoris. Methods Ultraviolet spectrophotometry was employed to detect content of total saponins from Rhizoma Panacis Majoris with detection wave length set at 540 nm. And effects of ethanol concentration,solvent quantity,extraction time and extraction times on extraction process were investigated by using orthogonal test design. Results Linear range of total saponins from Rhizoma Panacis Majoris was 0. 052 ~ 0. 182 mg,and the average recovery rate was 98. 64%. The best extraction technology for total saponins from Rhizoma Panacis Majoris was to extract 3 times with 10 times treatment of 70%ethanol for 1 h per time. The average yield rate of total saponins from Rhizoma Panacis Majoris was12. 32%. Conclusion This established ultraviolet spectrophotometry was accurate and reliable to detect contents of total saponins from Rhizoma Panacis Majoris. At the same time,this optimized extraction technology of total saponins from Rhizoma Panacis Majoris was simple,stable and feasible.

关 键 词：珠子参 总皂苷 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]