茵陈蒿汤血清药化学研究  

Studies on the serum pharmacochemistry of Yinchenhao Decoction

作　　者：许波[1] 窦志华[2] 罗琳[3] 顾薇[2] 孟萍[2] 刘青青[2] 王征宇[2] 

机构地区：[1]南京中医药大学药学院,江苏210023 [2]南京中医药大学南通中西医结合临床医学院 [3]南通大学药学院

出　　处：《北京中医药大学学报》2016年第2期162-168,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：江苏省中医药局科技项目(No.HZ07071);江苏省高校自然科学基金项目(No.08KJB360009)

摘　　要：目的采用血清药化学方法对茵陈蒿汤(YCHD)的入血成分进行分析。方法以SD大鼠为供体,灌胃给药制备YCHD及各缺味方、单味药含药血清,建立含药血清HPLC指纹图谱。与空白血清指纹图谱比对,指认含药血清中的药源性成分;与各缺味方、单味药含药血清比对,分析各药源性成分来源;与体外指纹图谱、对照品指纹图谱色谱峰保留时间及紫外吸收光谱图指纹图谱比对,对各药源性成分进行鉴别。结果 YCHD含药血清中检测到23个药源性成分,其中6个为体外原形成分,17个为体内代谢产物。6个原形成分分别为来源于栀子的京尼平苷酸、栀子苷、西红花酸类成分,来源于大黄的大黄酸及蒽醌类成分。17个代谢产物中的1个为环烯醚萜类成分代谢产物,7个为蒽醌类成分代谢产物,9个为西红花酸类成分代谢产物。结论 YCHD含药血清中的药源性成分主要为来源于大黄的蒽醌类成分、来源于栀子的环烯醚萜类成分和西红花酸类成分及以上成分的代谢产物,这些药源性成分可能构成了该方保肝作用的药效物质基础。Objective To analyze the constituents of Yinchenhao Decoction（ YCHD） absorbed into blood by serum pharmacochemistry. Methods SD rats were used as donor and serum containing drugs were prepared after intragastric administration of YCHD,YCHD without one of the component herbs,and individual herbs of YCHD. HPLC fingerprints of serum containing drug were established. By comparing the fingerprints of serum containing YCHD with the one of control serum,drug-induced constituents in blood were distinguished. By comparing the fingerprint of serum containing YCHD with the ones of serum containing YCHD without either one of the component herbs,and individual herbs of YCHD,the sources of drug-induced constituents were analyzed. By comparing the retention time and spectrograms of chromatographic peaks in fingerprint of serum containing YCHD with the ones of YCHD and reference substances,drug-induced constituents in blood were identified. Results 23 drug-induced constituents in serum containing YCHD were detected. 6 of them were original compounds,other 17 were metabolites. 3constituents out of 6 original compounds were identified as geniposidic acid and geniposide（ iridoids from Gardeniae Fructus）,and rhein（ anthraquinone from Rhei Radix et Rhizoma）,other three original compounds were identified as anthraquinone from Rhei Radix et Rhizoma（ RRR） and 2 crocetin derivatives from Gardeniae Fructus（ GF）. Out of 17 metabolites,1 was identified as the metabolite of iridoid,7 were identified as the metabolites of anthraquinone,and 9 were identified as the metabolites of crocetin derivatives. Conclusion Drug-induced constituents in serum containing YCHD include original form of anthraquinones from RRR,original form of iridoids and crocetin derivatives from GF,and the metabolites of the constituents mentioned above. All these drug-induced constituents may be the pharmacodynamic material basis for hepatoprotective effects of YCHD.

关 键 词：茵陈蒿汤 茵陈 栀子 大黄 含药血清 血清药化学 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]