Isolation of Prawn(Exopalaemon carinicauda) Lipopolysaccharide and β-1,3-Glucan Binding Protein Gene and Its Expression in Responding to Bacterial and Viral Infections  

作　　者：GE Qianqian LI Jian DUAN Yafei LI Jitao SUN Ming ZHAO Fazhen 

机构地区：[1]Fisheries College,Ocean University of China [2]Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture [3]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences [4]Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization of Ministry of Agriculture,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences

出　　处：《Journal of Ocean University of China》2016年第2期288-296,共9页中国海洋大学学报（英文版）

基　　金：supported by the earmarked fund for National ‘863’ Project of China (No.2012AA10A 409);Modern Agro-industry Technology Research System (No.CARS-47);Special Fund for Agro-scientific Research in the Public Interest (No.201103034);Independent Innovation Foundation of Shandong Province (No.2013CXC80202)

摘　　要：The pattern recognition proteins （PRPs） play a major role in immune response of crustacean to resist pathogens. In the present study, as one of PRPs, lipopolysaccharide and 13-1, 3-glucan binding protein （LGBP） gene in the ridge tail white prawn （Exopalaemon carinicauda） （EcLGBP） was isolated. The full-length cDNA of EcLGBP was 1338 bp, encoding a polypeptide of 366 amino acid residules. The deduced amino acid sequence of EcLGBP shared high similarities with LGBP and BGBP from other crus- taceans. Some conservative domains were predicted in EcLGBP sequence. EcLGBP constitutively expressed in most tissues at dif- ferent levels, and the highest expression was observed in hepatopancreas. With infection time, the cumulative mortality increased gradually followed by the proliferation of Vibrio parahaemolyticus and white spot syndrome virus （WSSV）. The expression of EcLGBP in response to E parahaemolyticus infection was up-regulated in hemocytes and hepatopancreas, and the up-regulation in hepatopancreas was earlier than that in hemocytes. EcLGBP expression after WSSV infection increased at 3 h, then significantly decreased in both hemocytes and hepatopancreas. The results indicated that EcLGBP was involved in the immune defense against bacterial and viral infections.The pattern recognition proteins(PRPs) play a major role in immune response of crustacean to resist pathogens.In the present study,as one of PRPs,lipopolysaccharide and β-1,3-glucan binding protein(LGBP) gene in the ridge tail white prawn(Exopalaemon carinicauda)(Ec LGBP) was isolated.The full-length c DNA of Ec LGBP was 1338 bp,encoding a polypeptide of 366 amino acid residules.The deduced amino acid sequence of Ec LGBP shared high similarities with LGBP and BGBP from other crustaceans.Some conservative domains were predicted in Ec LGBP sequence.Ec LGBP constitutively expressed in most tissues at different levels,and the highest expression was observed in hepatopancreas.With infection time,the cumulative mortality increased gradually followed by the proliferation of Vibrio parahaemolyticus and white spot syndrome virus(WSSV).The expression of Ec LGBP in response to V.parahaemolyticus infection was up-regulated in hemocytes and hepatopancreas,and the up-regulation in hepatopancreas was earlier than that in hemocytes.Ec LGBP expression after WSSV infection increased at 3 h,then significantly decreased in both hemocytes and hepatopancreas.The results indicated that Ec LGBP was involved in the immune defense against bacterial and viral infections.

关 键 词：Exopalaemon carinicauda LGBP WSSV Vibrio parahaemolyticus immune response 

分 类 号：S945.4[农业科学—水产养殖]