Characterization and Expression Analysis of B Cell Receptor Accessory Molecule CD79 Gene in Humphead Snapper(Lutjanus sanguineus)  

作　　者：HUANG Yucong YANXiuying CAI Shuanghu CAI Jia JIAN Jichang LU Yishan 3,4TANG Jufen WU Zaohe 

机构地区：[1]South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,P.R.China [2]University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]Fisheries College,Guangdong Ocean University,Zhanjiang 524088,P.R.China [4]Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals of Guangdong,Zhanjiang 524088,P.R.China [5]Zhongkai University of Agriculture and Engineering,Guangzhou 510225,P.R.China

出　　处：《Journal of Ocean University of China》2016年第2期318-326,共9页中国海洋大学学报（英文版）

基　　金：supported by the National Science&Technology Pillar Program of China(2012BAD17B02,2012BAD17B03);the National Natural Science Foundation of China(41240041,31402335)

摘　　要：CD79, a key component of the B cell antigen receptor complex, is composed of CD79a（Igα and CD7913（Igβ） encoded by rnb-1 and B29 respectively, and plays an important role in B cell signaling. In this study, we isolated and characterized rob-1 and B29 from humphead snapper （Lutjanus sanguineus）. Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated. The humphead snapper rob-1 and B29 contain open reading frames of 684 bp and 606 bp, encoding 227 amino acids and 201 amino acids, respectively. Both CD79α and CD79β possess signal peptide, extracellular Ig domain, transmem- brane region and immunoreceptor tyrosine kinase activation motif （ITAM）. Mb-1 is highly expressed in lymphoid organs （thymus, posterior kidney and spleen） and mucosal-associated lymphoid tissues （gill and intestine）, while B29 is mainly detected in posterior kidney, spleen, gill and skin. Furthermore, transcription of rob-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide （LPS）, pokeweed mitogen （PWM）, and polyinosinic-polycytidylic acid （PolyI：C） stimulation, respectively, and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi. These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection.CD79,a key component of the B cell antigen receptor complex,is composed of CD79α(Igα) and CD79β(Igβ) encoded by mb-1 and B29 respectively,and plays an important role in B cell signaling.In this study,we isolated and characterized mb-1 and B29 from humphead snapper(Lutjanus sanguineus).Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated.The humphead snapper mb-1 and B29 contain open reading frames of 684 bp and 606 bp,encoding 227 amino acids and 201 amino acids,respectively.Both CD79α and CD79β possess signal peptide,extracellular Ig domain,transmembrane region and immunoreceptor tyrosine kinase activation motif(ITAM).Mb-1 is highly expressed in lymphoid organs(thymus,posterior kidney and spleen) and mucosal-associated lymphoid tissues(gill and intestine),while B29 is mainly detected in posterior kidney,spleen,gill and skin.Furthermore,transcription of mb-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide(LPS),pokeweed mitogen(PWM),and polyinosinic-polycytidylic acid(Poly I:C) stimulation,respectively,and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi.These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection.

关 键 词：Lutjanus sanguineus immune response BCR CD79α CD79β 

分 类 号：S917.4[农业科学—水产科学]