Genetic Diversity Analysis of Glutinous Sorghum Germplasm Resources Based on SSR Markers  

利用SSR标记分析糯高粱种质资源的遗传多样性(英文)

作　　者：倪先林[1,2] 赵甘霖[1,2] 刘天朋[1,2] 龙文靖 胡炯凌[1,2] 丁国祥[1,2] 

机构地区：[1]四川省农业科学院水稻高粱研究所,四川德阳618000 [2]国家高粱改良中心四川分中心,四川泸州646000

出　　处：《Agricultural Science & Technology》2016年第3期499-504,共6页农业科学与技术（英文版）

基　　金：Supported by the Earmarked Fund for Modern Agro-industry Technology Research System(CARS-06-01-05);Secondary Subproject of the National Key Technology Research and Development Program during the 12~th Five-year Plan Period of China(2014BAD07B02-2-2);Key Project of Crop Breeding of Sichuang Province(2011NZ0098-6)~~

摘　　要：In order to evaluate the genetic relationships between glutinous sorghum germplasms, 25 pair of SSR markers were used to analyze the genetic diversity of29 varieties. The result showed that a total of 59 alleles were identified with an average of 2.28 alleles per locus. The average effective number of alleles（NE） was1.81.The average polymorphism information content（PIC） varied widely from 0.17 to0.62 with an average value of 0.34.The similarity coefficients of the 29 glutinous sorghum germplasms ranged from 0.203 to 0.949 with an average value of 0.593.Clustering analysis with UPGMA showed that the varieties could be divided into two groups at the genetic similarity coefficient of 0.475.The varieties from different geographic originswere clustered into one subclass, and the varieties with similar agronomic traits were clustered into one subclass, which indicated that the genetic relationships among materials was not in accordance with their geographical origins,and the glutinous sorghum germplasm resources showed abundant genetic diversity in the study.为了解析糯高粱种质资源之间的亲缘关系,用25对SSR引物检测了29份糯高粱种质资源的遗传多样性。结果表明,25对引物共检测出59个等位基因,平均每对引物检出2.28个,平均有效等位基因为1.81个,引物位点的多态信息量变幅为0.17～0.62,平均为0.34。29份糯高粱种质资源的遗传相似系数范围在0.203～0.949之间,平均为0.593,UPGMA方法将29份糯高粱品种在遗传相似系数0.475处聚为两大类。不同地理来源的品种被聚在同一亚类,农艺性状近似的品种聚在同一亚类。结果表明,糯高粱品种的亲缘关系与地理来源关系不大,该研究中的糯高粱种质资源具有较为丰富的遗传多样性。

关 键 词：SSR Glutinous sorghum Genetic diversity 

分 类 号：S514[农业科学—作物学]